Overview

  • Product name
  • Description
    Rabbit polyclonal to GRLF1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1200 and 1250 of human GRLF1 (EAW57452.1).

  • Positive control
    • HeLa and 293T whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab85950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 172 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.
    • Sequence similarities
      Contains 4 FF domains.
      Contains 1 Rho-GAP domain.
    • Post-translational
      modifications
      Tyrosine phosphorylated.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARHGAP35 antibody
      • GAP associated protein p190 antibody
      • Glucocorticoid receptor DNA binding factor 1 antibody
      • Glucocorticoid receptor DNA-binding factor 1 antibody
      • Glucocorticoid receptor repression factor 1 antibody
      • GRF 1 antibody
      • GRF-1 antibody
      • GRF1 antibody
      • GRLF 1 antibody
      • GRLF1 antibody
      • GRLF1_HUMAN antibody
      • KIAA1722 antibody
      • MGC10745 antibody
      • p190 A antibody
      • p190-A antibody
      • P190A antibody
      • P190A, rat, homolog of antibody
      • p190ARhoGAP antibody
      • p190RhoGAP antibody
      • Rho GAP p190A antibody
      • Rho GTPase-activating protein 35 antibody
      see all

    Images

    • All lanes : Anti-GRLF1 antibody (ab85950) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 172 kDa
      Observed band size : 172 kDa


      Exposure time : 10 seconds
    • Detection of human GRLF1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85950 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

      Detection: Chemiluminescence with an exposure time of 3 seconds.

    References

    ab85950 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab85950.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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