Anti-GRLF1 antibody (ab85950)
Key features and details
- Rabbit polyclonal to GRLF1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GRLF1 antibody
See all GRLF1 primary antibodies -
Description
Rabbit polyclonal to GRLF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human GRLF1 aa 1200-1300.
Database link: Q9NRY4 -
Positive control
- WB: HeLa, 293T, CT26-WT, 4T1 and Renca whole cell lysates. IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab85950 was affinity purified using an epitope specific to GRLF1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 172 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 172 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor. -
Sequence similarities
Contains 4 FF domains.
Contains 1 Rho-GAP domain. -
Post-translational
modificationsTyrosine phosphorylated.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2909 Human
- Entrez Gene: 232906 Mouse
- Entrez Gene: 306400 Rat
- Omim: 605277 Human
- SwissProt: Q9NRY4 Human
- SwissProt: Q91YM2 Mouse
- SwissProt: P81128 Rat
- Unigene: 509447 Human
see all -
Alternative names
- ARHGAP35 antibody
- GAP associated protein p190 antibody
- Glucocorticoid receptor DNA binding factor 1 antibody
see all
Images
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All lanes : Anti-GRLF1 antibody (ab85950) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 172 kDa
Observed band size: 172 kDa
Exposure time: 10 seconds -
All lanes : Anti-GRLF1 antibody (ab85950) at 1/2000 dilution
Lane 1 : CT26-WT (Mouse colon carcinoma cell - Wild Type) whole cell lysate
Lane 2 : 4T1 (Mouse mammary gland cells) whole cell lysate
Lane 3 : Renca (Mouse renal carcinoma cells) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 172 kDa
Exposure time: 30 seconds -
Detection of human GRLF1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85950 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab85950 has not yet been referenced specifically in any publications.