Anti-GRLF1 (phospho Y1105) antibody (ab55339)

Overview

  • Product name
    Anti-GRLF1 (phospho Y1105) antibody
    See all GRLF1 primary antibodies
  • Description
    Rabbit polyclonal to GRLF1 (phospho Y1105)
  • Specificity
    This antibody detects endogenous levels of GRLF1 only when phosphorylated at tyrosine 1105.
  • Tested applications
    Suitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human)(Peptide available as ab106616.)

  • Positive control
    • IHC-P: Human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitopespecific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • Function
    Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.
  • Sequence similarities
    Contains 4 FF domains.
    Contains 1 Rho-GAP domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGAP35 antibody
    • GAP associated protein p190 antibody
    • Glucocorticoid receptor DNA binding factor 1 antibody
    • Glucocorticoid receptor DNA-binding factor 1 antibody
    • Glucocorticoid receptor repression factor 1 antibody
    • GRF 1 antibody
    • GRF-1 antibody
    • GRF1 antibody
    • GRLF 1 antibody
    • GRLF1 antibody
    • GRLF1_HUMAN antibody
    • KIAA1722 antibody
    • MGC10745 antibody
    • p190 A antibody
    • p190-A antibody
    • P190A antibody
    • P190A, rat, homolog of antibody
    • p190ARhoGAP antibody
    • p190RhoGAP antibody
    • Rho GAP p190A antibody
    • Rho GTPase-activating protein 35 antibody
    see all

Anti-GRLF1 (phospho Y1105) antibody images

  • ab55339, at 1/50 dilution, staining GRLF1 in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising phosphopeptide.

References for Anti-GRLF1 (phospho Y1105) antibody (ab55339)

ab55339 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (kidney)
Loading amount
60 µg
Specification
kidney
Gel Running Conditions
Reduced Denaturing (7,5%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Sep 07 2009

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