SpecificityBased on direct ELISA and immunoblotting results, shows less than 60% cross-reactivity with human GRO beta and less than 30% cross-reactivity with human GRO gamma.
Shows no cross-reactivity with other cytokines tested using direct ELISA. Because this is a total IgG fraction, complete monospecificity cannot be assumed.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use at an assay dependent dilution. Predicted molecular weight: 11.3 kDa.
FunctionHas chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
Post-translational modificationsN-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.