Overview

  • Product name
  • Description
    Rabbit polyclonal to GRO alpha
  • Specificity
    The immunogen for ab86436 has >90% homology to mouse GRO beta
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide surrounding amino acid 78 of mouse GRO alpha.

  • Positive control
    • Jurkat and 3T3 cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 30% Glycerol, 0.5% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa).

Target

  • Function
    Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C motif chemokine 1 antibody
    • Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antibody
    • chemokine (C-X-C motif) ligand 1 antibody
    • CINC-1 antibody
    • CXCL1 antibody
    • Cytokine-induced neutrophil chemoattractant 1 antibody
    • Fibroblast secretory protein antibody
    • Fsp antibody
    • Gro 1 antibody
    • Gro A antibody
    • Gro antibody
    • GRO protein, alpha antibody
    • GRO-alpha(1-73) antibody
    • GRO-alpha(6-73) antibody
    • Gro1 antibody
    • GRO1 oncogene (melanoma growth stimulating activity, alpha) antibody
    • GRO1 oncogene (melanoma growth-stimulating activity) antibody
    • Gro1 oncogene antibody
    • GROa antibody
    • GROA_HUMAN antibody
    • Growth-regulated alpha protein antibody
    • KC antibody
    • KC chemokine, mouse, homolog of antibody
    • melanoma growth stimulatory activity alpha antibody
    • Melanoma growth stimulatory activity antibody
    • Melanoma growth stimulatory activity, alpha antibody
    • MGSA alpha antibody
    • MGSA antibody
    • MGSA-a antibody
    • N51 antibody
    • NAP-3 antibody
    • NAP3 antibody
    • Neutrophil-activating protein 3 antibody
    • Platelet-derived growth factor-inducible protein KC antibody
    • Scyb 1 antibody
    • Scyb1 antibody
    • Secretory protein N51 antibody
    • Small inducible cytokine subfamily B, member 1 antibody
    see all

Anti-GRO alpha antibody images

  • ICC/IF image of ab86436 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86436, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-GRO alpha antibody (ab86436) at 0.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : 3T3 cell lysate


    Predicted band size : 11 kDa
    Observed band size : 9 kDa (why is the actual band size different from the predicted?)

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 11 kDa


    Exposure time : 1 minute

References for Anti-GRO alpha antibody (ab86436)

This product has been referenced in:
  • Wan XY  et al. Antimetastatic effects of norcantharidin on hepatocellular carcinoma cells by up-regulating FAM46C expression. Am J Transl Res 9:155-166 (2017). WB ; Human . Read more (PubMed: 28123642) »
  • Leng Y  et al. Effects of acute intra-abdominal hypertension on multiple intestinal barrier functions in rats. Sci Rep 6:22814 (2016). Read more (PubMed: 26980423) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Tissue sections (Intervertebral Discs))
Specification
Human Tissue sections (Intervertebral Discs)
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris Buffer pH:9,5 for 2x5’ in MW 40% power
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 22°C
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Verified customer

Submitted Apr 11 2012

Thank you for contacting us today.

I am very pleased to hear you would like to test ab86436, ab9841and ab10064with IHC ofparaffin embedded human tissue sections. The codes below will each give you 1 freeprimary antibodybefore the expiration d...

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