Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898)

Overview

  • Product nameAnti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902]
  • Description
    Rabbit monoclonal [EPR4902] to GRO alpha + GRO gamma + GRO beta
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GRO alpha aa 50-150.

  • Positive control
    • Human GRO alpha recombinant protein.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR4902
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 8 kDa (predicted molecular weight: 11 kDa).
  • Application notesIs unsuitable for ICC/IF,IHC-P or IP.
  • Target

    Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] images

    • All lanes : Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898) at 1/2000 dilution

      Lane 1 : Human GRO alpha active protein
      Lane 2 : Human GRO beta fragment recombinant protein
      Lane 3 : Human GRO gamma fragment recombinant protein

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1 & 3 : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/100000 dilution
      Lane 2 : Goat Ant-Rabbit IgG,(H+L),Peroxidase conjugated at 1/100000 dilution


      Predicted band size : 11 kDa
      Observed band size : kDa (why is the actual band size different from the predicted?)

      Exposure time: 3 minutes

      Blocking buffer and concentartion:5% NFDM/TBST

      Diluting buffer and concentration:5% NFDM/TBST

      Human GRO beta fragment pritein contain aa35-107 with a GST-Tage.Human GRO gamma fragment recombinant protein contain aa35-107 with a His-Tag.

    References for Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898)

    ab124898 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"