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Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902]
Rabbit monoclonal [EPR4902] to GRO alpha + GRO gamma + GRO beta
WB more details Unsuitable for:
ICC/IF,IHC-P or IP
Synthetic peptide within Human GRO alpha aa 50-150. The exact sequence is proprietary.
Human GRO alpha recombinant protein.
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide Constituents: 50% Glycerol, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 8 kDa (predicted molecular weight: 11 kDa).
Is unsuitable for ICC/IF,IHC-P or IP.
GRO alpha: Secreted.
GRO gamma: Secreted.
Western blot - Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898)
All lanes :
Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898) at 1/2000 dilution
Lane 1 :
Human GRO alpha active protein
Lane 2 :
Human GRO beta fragment recombinant protein
Lane 3 :
Human GRO gamma fragment recombinant protein
Lysates/proteins at 10 µg per lane.
Secondary Lanes 1 & 3 :
Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/100000 dilution
Lane 2 :
Goat Ant-Rabbit IgG,(H+L),Peroxidase conjugated at 1/100000 dilution
Predicted band size:
Observed band size:
10,36,10 kDa (
why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking buffer and concentartion:5% NFDM/TBST
Diluting buffer and concentration:5% NFDM/TBST
Human GRO beta fragment pritein contain aa35-107 with a GST-Tage.Human GRO gamma fragment recombinant protein contain aa35-107 with a His-Tag.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"