Anti-GRO gamma antibody (ab10064)

Overview

  • Product nameAnti-GRO gamma antibody
    See all GRO gamma primary antibodies
  • Description
    Rabbit polyclonal to GRO gamma
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 85-99 of Human CXCL3.

  • Positive control
    • HeLa whole cell lysate.
  • General notes


    CXCL3 may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, Sucrose
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesCXCL3 may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 12.7 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionLigand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C motif chemokine 3 antibody
    • Chemokine (C X C motif) ligand 3 antibody
    • Chemokine (CXC motif) ligand 3 antibody
    • Cinc 2 antibody
    • CINC 2b antibody
    • Cinc2 antibody
    • CINC2b antibody
    • CXCL 3 antibody
    • Cxcl3 antibody
    • CXCL3_HUMAN antibody
    • Cytokine induced neutrophil chemoattractant 2 antibody
    • Dcip1 antibody
    • Dendritic cell inflammatory protein 1 antibody
    • Gm1960 antibody
    • GRO protein gamma antibody
    • GRO-gamma antibody
    • GRO-gamma(1-73) antibody
    • GRO-gamma(5-73) antibody
    • GRO3 antibody
    • GRO3 oncogene antibody
    • GROG antibody
    • Growth regulated protein gamma antibody
    • Growth-regulated protein gamma antibody
    • Macrophage inflammatory protein 2 beta precursor antibody
    • Macrophage inflammatory protein 2-beta antibody
    • Melanoma growth stimulatory activity gamma antibody
    • Member 3 antibody
    • MGSA gamma antibody
    • MIP 2b antibody
    • MIP2-beta antibody
    • MIP2B antibody
    • SCYB3 antibody
    • Small inducible cytokine subfamily B antibody
    see all

Anti-GRO gamma antibody images



  • Predicted band size : 12.7 kDa


    ab10064 at 1/1000 dilution staining approximately 11kDa band of human CXCL3 in HeLa whole cell lysate by Western blot (ECL).

References for Anti-GRO gamma antibody (ab10064)

ab10064 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Tissue sections (Intervertebral Discs))
Specification Human Tissue sections (Intervertebral Discs)
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris Buffer pH:9,5 for 2x5’ in MW 40% power
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 22°C
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Submitted Apr 11 2012

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I am very pleased to hear you would like to test ab86436, ab9841and ab10064with IHC ofparaffin embedded human tissue sections. The codes below will each give you 1 freeprimary antibodybefore the expiration d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"