Overview

  • Product nameAnti-GRO gamma antibody
    See all GRO gamma primary antibodies
  • Description
    Goat polyclonal to GRO gamma
  • SpecificityIn ELISA assays, anti-CINC2 beta exhibits the following cross-reactivities: rratCINC2 alpha 100%, rmouseMIP2 50%, rmouse KC 35%, rrat CINC1 15%, rhuman IL8 10%, rhuman GRO alpha 10%, rhuman GRO beta 10%, rhuman GRO gamma 10%.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Purified recombinant rat CINC-2beta expressed in E. coli.

  • General notesEndotoxin: <10 ng/mg antibody determined by the LAL method.


    Rat CINC2 beta (Cytokine Induced Neutrophil Chemoattractant 2 beta) is a member of the CXC subfamily of chemokines which also includes three other rat CXC chemokines (CINC1, CINC2 alpha, CINC3/MIP2). The rat chemokines CINC show sequence similarity to human GROs and mouse MIP2 but not IL8. Mature CINC2 beta shares 63% amino acid sequence identity with CINC1 and 80% identity to CINC3. Rat CINC2 beta cDNA encodes a 68 amino acid residue protein. The mature recombinant protein has a predicted molecular mass of approximately 7.6 kDa. Based on sequence homology, it has been suggested that CINCs are the rat counterpart of the human GROs. CINC2 alpha and CINC2 beta were originally purified as novel neutrophil chemoattractants from the conditioned medium of rat granulation tissue CINC2 proteins represent the major chemokines purified from conditioned medium of granulation tissue or lipopolysaccharide (LPS) induced inflammatory exudate, though CINC1 and CINC3 are also present. Other cell types known to produce CINC2 proteins include activated macrophages and fibroblasts. Rat CINCs, potent neutrophil attractants and activators, have an important role in the infiltration of neutrophils into inflammatory sites in rats. Recombinant and natural CINC2 proteins are specific neutrophil chemoattractants both in vivo and in vitro. On the basis of cross-desensitization, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC3 specific receptor as well as a second common receptor shared by all CINCs.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity isolated antigen specific antibody is obtained from goat anti-CINC2 beta antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the peptide.
  • Primary antibody notesRat CINC2 beta (Cytokine Induced Neutrophil Chemoattractant 2 beta) is a member of the CXC subfamily of chemokines which also includes three other rat CXC chemokines (CINC1, CINC2 alpha, CINC3/MIP2). The rat chemokines CINC show sequence similarity to human GROs and mouse MIP2 but not IL8. Mature CINC2 beta shares 63% amino acid sequence identity with CINC1 and 80% identity to CINC3. Rat CINC2 beta cDNA encodes a 68 amino acid residue protein. The mature recombinant protein has a predicted molecular mass of approximately 7.6 kDa. Based on sequence homology, it has been suggested that CINCs are the rat counterpart of the human GROs. CINC2 alpha and CINC2 beta were originally purified as novel neutrophil chemoattractants from the conditioned medium of rat granulation tissue CINC2 proteins represent the major chemokines purified from conditioned medium of granulation tissue or lipopolysaccharide (LPS) induced inflammatory exudate, though CINC1 and CINC3 are also present. Other cell types known to produce CINC2 proteins include activated macrophages and fibroblasts. Rat CINCs, potent neutrophil attractants and activators, have an important role in the infiltration of neutrophils into inflammatory sites in rats. Recombinant and natural CINC2 proteins are specific neutrophil chemoattractants both in vivo and in vitro. On the basis of cross-desensitization, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC3 specific receptor as well as a second common receptor shared by all CINCs.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 7.6 kDa.

The detection limit of rat CINC2 beta is approximately 5 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively.

ELISA Use a concentration of 0.5 - 1 µg/ml.

The detection limit for rat CINC2 beta is approximately 0.5 ng/well.

Target

  • FunctionLigand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C motif chemokine 3 antibody
    • Chemokine (C X C motif) ligand 3 antibody
    • Chemokine (CXC motif) ligand 3 antibody
    • Cinc 2 antibody
    • CINC 2b antibody
    • Cinc2 antibody
    • CINC2b antibody
    • CXCL 3 antibody
    • Cxcl3 antibody
    • CXCL3_HUMAN antibody
    • Cytokine induced neutrophil chemoattractant 2 antibody
    • Dcip1 antibody
    • Dendritic cell inflammatory protein 1 antibody
    • Gm1960 antibody
    • GRO protein gamma antibody
    • GRO-gamma antibody
    • GRO-gamma(1-73) antibody
    • GRO-gamma(5-73) antibody
    • GRO3 antibody
    • GRO3 oncogene antibody
    • GROG antibody
    • Growth regulated protein gamma antibody
    • Growth-regulated protein gamma antibody
    • Macrophage inflammatory protein 2 beta precursor antibody
    • Macrophage inflammatory protein 2-beta antibody
    • Melanoma growth stimulatory activity gamma antibody
    • Member 3 antibody
    • MGSA gamma antibody
    • MIP 2b antibody
    • MIP2-beta antibody
    • MIP2B antibody
    • SCYB3 antibody
    • Small inducible cytokine subfamily B antibody
    see all

References for Anti-GRO gamma antibody (ab10367)

ab10367 has not yet been referenced specifically in any publications.

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