Overview

  • Product name
  • Description
    Rabbit polyclonal to GRO gamma
  • Tested applications
    Suitable for: WB, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hGROg (human GRO-gamma)

Properties

  • Form
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Neutralising
  • Application notes
    Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hGROg (100.00 ng/ml), a concentration of 1.0 - 2.0 µg/ml of this antibody is required.

    ELISA:To detect hGROg by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGROg.

    Western Blot:To detect hGROg by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGROg is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Target

    • Function
      Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.
    • Sequence similarities
      Belongs to the intercrine alpha (chemokine CxC) family.
    • Post-translational
      modifications
      N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-X-C motif chemokine 3 antibody
      • C-X-C motif chemokine ligand 3 antibody
      • Chemokine (C X C motif) ligand 3 antibody
      • Chemokine (CXC motif) ligand 3 antibody
      • Cinc 2 antibody
      • CINC 2b antibody
      • Cinc2 antibody
      • CINC2b antibody
      • CXCL 3 antibody
      • Cxcl3 antibody
      • CXCL3_HUMAN antibody
      • Cytokine induced neutrophil chemoattractant 2 antibody
      • Dcip1 antibody
      • Dendritic cell inflammatory protein 1 antibody
      • Gm1960 antibody
      • GRO protein gamma antibody
      • GRO-gamma antibody
      • GRO-gamma(1-73) antibody
      • GRO-gamma(5-73) antibody
      • GRO3 antibody
      • GRO3 oncogene antibody
      • GROG antibody
      • Growth regulated protein gamma antibody
      • Growth-regulated protein gamma antibody
      • Macrophage inflammatory protein 2 beta precursor antibody
      • Macrophage inflammatory protein 2-beta antibody
      • Melanoma growth stimulatory activity gamma antibody
      • Member 3 antibody
      • MGSA gamma antibody
      • MIP 2b antibody
      • MIP2-beta antibody
      • MIP2B antibody
      • SCYB3 antibody
      • Small inducible cytokine subfamily B antibody
      see all

    Images

    • Anti-GRO gamma antibody (ab9843) at 1 µg/ml + Recombinant Human GRO gamma protein (ab92858) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 10 seconds

    References

    ab9843 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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