Overview

  • Product name
  • Description
    Rabbit polyclonal to groEL
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    E. coli groEL protein

Properties

Applications

Our Abpromise guarantee covers the use of ab90522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 57 kDa.

Target

  • Relevance
    The bacterial chaperonin groEL is a double toroidal assembly, which together with the action of the ring-shaped oligomeric cochaperonin, GroES, facilitates protein folding in an ATP dependent manner.
  • Cellular localization
    Cytoplasmic
  • Database links
    • Alternative names
      • 60 kDa chaperonin antibody
      • groEL protein antibody
      • groL antibody
      • mopA antibody
      • Protein Cpn60 antibody
      see all

    References

    This product has been referenced in:
    • Lynch S  et al. Overcoming substrate limitations for improved production of ethylene in E. coli. Biotechnol Biofuels 9:3 (2016). WB ; Escherichia coli . Read more (PubMed: 26734073) »
    • Oron-Gottesman A  et al. A Stress-Induced Bias in the Reading of the Genetic Code in Escherichia coli. MBio 7:N/A (2016). Read more (PubMed: 27935840) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Pseudomonas aeruginosa Cell lysate - whole cell (pseudomonas aeruginosa)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    1e+006 cells
    Specification
    pseudomonas aeruginosa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Yu Zhang

    Verified customer

    Submitted Jan 12 2016

    Application
    Western blot
    Loading amount
    75 µg
    Gel Running Conditions
    Reduced Denaturing (18)
    Sample
    Escherichia coli Cell lysate - whole cell (E. coli Deletion variants)
    Specification
    E. coli Deletion variants
    Treatment
    20 mM Nitrat, no oxygen for 16 hrs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 14 2014


    I am very pleased to hear you would like to accept our offer and test ab90522 in P. aerugionsa. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for P. aerugionsa and incl...

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    Thank you very much for your interest in ab90522.

    To our knowledge, ab90522 has not been tested in P. aerugionsa. Therefore, I can offer a discount off a future purchase if you buy ab90522 now, test it in P. aerugionsa and submit feedback to u...

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    Thank you for contacting Abcam regarding ab90522.


    We have not tested this cross reactivity specifically so I did an alignment. Based on a sequence alignment in Uniprot, there is only about 60% homology between various strains of E. Coli...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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