• Product nameAnti-groEL antibody
    See all groEL primary antibodies
  • Description
    Rabbit polyclonal to groEL
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    E. coli groEL protein



Our Abpromise guarantee covers the use of ab90522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 57 kDa.


  • RelevanceThe bacterial chaperonin groEL is a double toroidal assembly, which together with the action of the ring-shaped oligomeric cochaperonin, GroES, facilitates protein folding in an ATP dependent manner.
  • Cellular localizationCytoplasmic
  • Database links
    • Alternative names
      • 60 kDa chaperonin antibody
      • groEL protein antibody
      • groL antibody
      • mopA antibody
      • Protein Cpn60 antibody
      see all

    References for Anti-groEL antibody (ab90522)

    This product has been referenced in:
    • Sabater-Muñoz B  et al. Fitness trade-offs determine the role of the molecular chaperonin GroEL in buffering mutations. Mol Biol Evol N/A:N/A (2015). Read more (PubMed: 26116858) »
    • Feiler C  et al. Directed evolution of Mycobacterium tuberculosis ß-lactamase reveals gatekeeper residue that regulates antibiotic resistance and catalytic efficiency. PLoS One 8:e73123 (2013). WB ; Escherichia coli . Read more (PubMed: 24023821) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Sample Pseudomonas aeruginosa Cell lysate - whole cell (pseudomonas aeruginosa)
    Gel Running Conditions Non-reduced Denaturing
    Loading amount 1e+006 cells
    Specification pseudomonas aeruginosa
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Yu Zhang

    Verified customer

    Submitted Jan 12 2016

    Application Western blot
    Loading amount 75 µg
    Gel Running Conditions Reduced Denaturing (18)
    Sample Escherichia coli Cell lysate - whole cell (E. coli Deletion variants)
    Specification E. coli Deletion variants
    Treatment 20 mM Nitrat, no oxygen for 16 hrs
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Oct 14 2014

    I am very pleased to hear you would like to accept our offer and test ab90522 in P. aerugionsa. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for P. aerugionsa and incl...

    Read More

    Thank you very much for your interest in ab90522.

    To our knowledge, ab90522 has not been tested in P. aerugionsa. Therefore, I can offer a discount off a future purchase if you buy ab90522 now, test it in P. aerugionsa and submit feedback to u...

    Read More

    Thank you for contacting Abcam regarding ab90522.

    We have not tested this cross reactivity specifically so I did an alignment. Based on a sequence alignment in Uniprot, there is only about 60% homology between various strains of E. Coli...

    Read More

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

    Read More