Anti-groES antibody (ab69823)
Key features and details
- Rabbit polyclonal to groES
- Suitable for: WB, IP
- Reacts with: Escherichia coli
- Isotype: IgG
Overview
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Product name
Anti-groES antibody -
Description
Rabbit polyclonal to groES -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Full length protein corresponding to Escherichia coli groES. Strain K12
Database link: P0A6F9 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). for colorimetric detection. 1/500 for ECL.
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IP |
1/200.
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Notes |
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WB
1/5000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). for colorimetric detection. 1/500 for ECL. |
IP
1/200. |
Target
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Relevance
GroEL and GroES are E.coli chaperones, which are homologs of the mitochondrial chaperones Hsp60 and Hsp10 respectively. GroEL is a double toriodal assembly of 14 identical subunits which form two heptameric rings stacked back-to-back, with a cavity at each end. GroEL and its co-chaperonin GroES facilitate protein folding in an ATP-dependent mechanism. Binding of substrate protein, in addition to binding of ATP, induces a conformational change that allows association of this binary complex with a separate lid structure, GroES. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 959981 Escherichia coli
- SwissProt: P0A6G1 Escherichia coli
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Alternative names
- 10 kDa chaperonin antibody
- Co-chaperonin GroES antibody
- groES protein antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab69823 has been referenced in 4 publications.
- Lagha R et al. Effect of Plasmonic Gold Nanoprisms on Biofilm Formation and Heat Shock Proteins Expression in Human Pathogenic Bacteria. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 34959736
- Sabater-Muñoz B et al. Fitness trade-offs determine the role of the molecular chaperonin GroEL in buffering mutations. Mol Biol Evol N/A:N/A (2015). PubMed: 26116858
- Cimdins A et al. Differential control of Salmonella heat shock operons by structured mRNAs. Mol Microbiol 89:715-31 (2013). PubMed: 23802546
- Zhou Y & Chen WN iTRAQ-coupled 2-D LC-MS/MS analysis of cytoplasmic protein profile in Escherichia coli incubated with apidaecin IB. J Proteomics : (2011). WB ; Escherichia coli . PubMed: 21889622