Anti-Growth Cone antibody [2G13] (ab7762)
Key features and details
- Mouse monoclonal [2G13] to Growth Cone
- Suitable for: WB, IHC-Fr, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Chicken
- Isotype: IgM
Overview
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Product name
Anti-Growth Cone antibody [2G13] -
Description
Mouse monoclonal [2G13] to Growth Cone -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Fr, ICC/IF, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Chicken -
Immunogen
Tissue, cells or virus corresponding to Chicken Growth Cone. Embryonic chick tectal membranes
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General notes
This antibody is useful as a general reagent for the detection of axonal growth cones in tissue sections and potentially a key marker in neuronal development research.
This antibody recognises an intracellular protein 2G13p which is restricted to growth cones in developing brain and embryonic neurons in culture. The protein 2G13p also appears to be associated with filamentous actin in the growth cones suggesting a role for 2G13p in growth cone motility. This antibody is suitable for use in immunohistological studies of both frozen and paraffin-embedded sections, staining growth cones, including their filopodia and lamellipodies.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Tissue culture supernatant -
Primary antibody notes
This antibody recognises an intracellular protein 2G13p which is restricted to growth cones in developing brain and embryonic neurons in culture. The protein 2G13p also appears to be associated with filamentous actin in the growth cones suggesting a role for 2G13p in growth cone motility. This antibody is suitable for use in immunohistological studies of both frozen and paraffin-embedded sections, staining growth cones, including their filopodia and lamellipodies. -
Clonality
Monoclonal -
Clone number
2G13 -
Myeloma
NS1 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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IHC-P |
1/4.
This product does not require protein digestion pre-treatment sections e.g. trypsin or pronase, and does not require antigen retrieval using heat treatment prior to staining. |
Notes |
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WB
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
1/4. This product does not require protein digestion pre-treatment sections e.g. trypsin or pronase, and does not require antigen retrieval using heat treatment prior to staining. |
Target
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Relevance
Growth cones are specialised sensory structures located at the tip of neurites which are involved in the mechanism that enable elongation and navigation of axons during neuronal development.
Images
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab7762 has been referenced in 4 publications.
- Smith LK et al. Direct interaction of HIV gp120 with neuronal CXCR4 and CCR5 receptors induces cofilin-actin rod pathology via a cellular prion protein- and NOX-dependent mechanism. PLoS One 16:e0248309 (2021). PubMed: 33705493
- Kumar H et al. Elevated TRPV4 Levels Contribute to Endothelial Damage and Scarring in Experimental Spinal Cord Injury. J Neurosci 40:1943-1955 (2020). PubMed: 31974206
- Wülfing C & Günther HS Dendritic cells and macrophages neurally hard-wired in the lymph node. Sci Rep 5:16866 (2015). PubMed: 26581550
- Stettler O et al. Monoclonal antibody 2G13, a new axonal growth cone marker. J Neurocytol 28:1035-44 (1999). PubMed: 11054903