Overview

  • Product nameAnti-Growth Hormone antibody [GH-1]
    See all Growth Hormone primary antibodies
  • Description
    Mouse monoclonal [GH-1] to Growth Hormone
  • Tested applicationsELISA, Indirect ELISA, WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • EpitopeAb9821 recognizes a different epitope than GH-2 (ab9822).

Properties

Applications

Our Abpromise guarantee covers the use of ab9821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/250.
Indirect ELISA 1/1000 - 1/10000.
WB 1/500 - 1/2000. Predicted molecular weight: 22-24 kDa.
Sandwich ELISA Use at an assay dependent dilution.

Target

  • FunctionPlays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • Involvement in diseaseDefects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
    Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
  • Sequence similaritiesBelongs to the somatotropin/prolactin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • GH 1 antibody
    • GH antibody
    • GH N antibody
    • GH-N antibody
    • GH1 antibody
    • GHN antibody
    • GHN antibody
    • Growth Hormone 1 antibody
    • Growth hormone 1 variant 1 antibody
    • Growth hormone 1 variant 2 antibody
    • Growth hormone antibody
    • Growth Hormone Normal antibody
    • Growth Hormone Pituitary antibody
    • Growth hormone, normal antibody
    • Growth hormone, pituitary antibody
    • HG1 antibody
    • HG1 antibody
    • hGH N antibody
    • hGH-N antibody
    • HGHN antibody
    • IGHD1B antibody
    • Pituitary Growth Hormone antibody
    • RNGHGP antibody
    • SOMA_HUMAN antibody
    • Somatotropin C antibody
    • Somatotropin antibody
    • Somatotropin I Precursor antibody
    see all

Anti-Growth Hormone antibody [GH-1] images

  • All lanes : Anti-Growth Hormone antibody [GH-1] (ab9821) at 1/500 dilution

    Lane 1 : Human Human Growth Hormone full length protein (ab51232) at 0.1 µg
    Lane 2 : Human Human Growth Hormone full length protein (ab51232) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes


  • Predicted band size : 22-24 kDa


    Western blot of human growth hormone using ab9821 at a concentration of 1 µg/ml.

References for Anti-Growth Hormone antibody [GH-1] (ab9821)

This product has been referenced in:
  • Rezaei B  et al. Immobilization of specific monoclonal antibody on Au nanoparticles for hGH detection by electrochemical impedance spectroscopy. Biosens Bioelectron 25:395-9 (2009). Read more (PubMed: 19692224) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry, we have received the following information from the source of those antibodies: Monoclonal antibodies ab9821 and 9822 were raised against the recombinant human growth hormone (hGH) cloned from mRNA (GenBank ac. No NM-0005...

Read More

Binding capacity in serum was not determined for ab9821.

You get 100 micro-litres for £195.00 sterling.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"