Overview

  • Product nameAnti-Growth Hormone antibody [GH-45]
    See all Growth Hormone primary antibodies
  • Description
    Mouse monoclonal [GH-45] to Growth Hormone
  • SpecificitySpecifically reacts with human growth hormone with affinity constant 3.8 x 1010 l /mol. It does not bind human prolactin or any other pituitary hormones.
  • Tested applicationsSuitable for: ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified from ascites using protein A-affinity chromatography Purity >95 % (by PAGE).
  • ClonalityMonoclonal
  • Clone numberGH-45
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
IHC-P
  • Application notesICC: Use at an assay dependant dilution.
    IHC-P: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
    • Involvement in diseaseDefects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
      Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
      Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
      Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
    • Sequence similaritiesBelongs to the somatotropin/prolactin family.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • gH antibody
      • GH-N antibody
      • GH1 antibody
      • GHN antibody
      • Growth hormone 1 antibody
      • Growth hormone antibody
      • Growth hormone, normal antibody
      • Growth hormone, pituitary antibody
      • HG1 antibody
      • hGH-N antibody
      • IGHD1B antibody
      • Pituitary growth hormone antibody
      • RNGHGP antibody
      • SOMA_HUMAN antibody
      • Somatotropin antibody
      see all

    Anti-Growth Hormone antibody [GH-45] images

    • Human normal brain (pituitary gland). Staining is observed in the cytoplasm and in the extracellulat space. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    References for Anti-Growth Hormone antibody [GH-45] (ab7905)

    This product has been referenced in:
    • Ho Y  et al. Distinct chromatin configurations regulate the initiation and the maintenance of hGH gene expression. Mol Cell Biol 33:1723-34 (2013). Read more (PubMed: 23428872) »
    • Barbieri F  et al. Overexpression of stromal cell-derived factor 1 and its receptor CXCR4 induces autocrine/paracrine cell proliferation in human pituitary adenomas. Clin Cancer Res 14:5022-32 (2008). IHC-P ; Human . Read more (PubMed: 18698020) »

    See all 2 Publications for this product

    Product Wall

    I have enquired with the suppliers of the other Growth hormone antibodies that we carry, and as for ab7905 the supplier informed me that the antibody reacts with human growth hormone with affinity constant 3.8 x 10E10 l /mol. I am still waiting to he...

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    I would not recommend the use of an antibody raised in mouse for use in IHC on mouse tissue. This is because the anti-mouse secondary would also detect endogenous mouse IgGs in the tissue. ab8490 -- Rabbit polyclonal to human Growth Hormone -- has been...

    Read More

    This antibody has not been tested in rats. Currently we do not stock any other antibodies to growth hormones

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"