Anti-Growth hormone receptor antibody (ab65304)

Overview

  • Product nameAnti-Growth hormone receptor antibody
    See all Growth hormone receptor primary antibodies
  • Description
    Rabbit polyclonal to Growth hormone receptor
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Growth hormone receptor.

    (Peptide available as ab73395.)

  • Positive control
    • This antibody gave a positive result in the following whole cell lysates: A549, HeLa, DU 145

Properties

Applications

Our Abpromise guarantee covers the use of ab65304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 71 kDa).

Target

  • FunctionReceptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway.
    The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
    Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling.
  • Tissue specificityExpressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 is expressed in lung, stomach and muscle. Low levels in liver.
  • Involvement in diseaseDefects in GHR are a cause of Laron syndrome (LARS) [MIM:262500]. A severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone.
    Defects in GHR may be a cause of idiopathic short stature autosomal (ISSA) [MIM:604271]. Short stature is defined by a subnormal rate of growth.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 1 fibronectin type-III domain.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
    The extracellular domain is the ligand-binding domain representing the growth hormone-binding protein (GHBP).
    The ubiquitination-dependent endocytosis motif (UbE) is required for recruitment of the ubiquitin conjugation system on to the receptor and for its internalization.
  • Post-translational
    modifications
    The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17.
    On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2.
    On ligand binding, ubiquitinated on lysine residues in the cytoplasmic domain. This ubiquitination is not sufficient for GHR internalization.
  • Cellular localizationSecreted; Cell membrane. On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway and Cell membrane. Remains fixed to the cell membrane and is not internalized.
  • Information by UniProt
  • Database links
  • Alternative names
    • GH receptor antibody
    • GH-binding protein antibody
    • GHBP antibody
    • GHBP, included antibody
    • GHR antibody
    • GHR_HUMAN antibody
    • Growth hormone binding protein antibody
    • Growth hormone receptor antibody
    • Growth hormone receptor precursor antibody
    • Growth hormone-binding protein antibody
    • Growth hormone-binding protein, included antibody
    • Increased responsiveness to growth hormone, included antibody
    • Serum binding protein antibody
    • Serum-binding protein antibody
    • Somatotropin receptor antibody
    see all

Anti-Growth hormone receptor antibody images

  • All lanes : Anti-Growth hormone receptor antibody (ab65304) at 1 µg/ml

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 71 kDa
    Observed band size : 76 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 56 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
    Growth hormone receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ICC/IF image of ab65304 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65304, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.

References for Anti-Growth hormone receptor antibody (ab65304)

This product has been referenced in:
  • Beltran-Sastre V  et al. Tuneable endogenous mammalian target complementation via multiplexed plasmid-based recombineering. Sci Rep 5:17432 (2015). WB ; Human . Read more (PubMed: 26612112) »

See 1 Publication for this product

Product Wall

Growth hormone receptor is about 72 kDa before any post-translation modifications. (See the section titled Sequences, halfway down the page of the UniProt record for human GHR at this link: http://www.uniprot.org/uniprot/P10912 ).

But subseque...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Glioblastoma)
Specification Glioblastoma
Fixative Paraformaldehyde
Permeabilization Yes - TBS Tween 0.5%
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. Maïté Verreault

Verified customer

Submitted May 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Glioblastoma)
Loading amount 30 µg
Specification Glioblastoma
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Pierce TBS Super block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 02 2013

Thank you for contacting us. The immunogen sequences for these antibodies are considered proprietary, but I can tell you that for both ab65304 and ab78426 the sequences are from human and do not overlap. Ab65304 was raised against an immunogen from wit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"