Anti-Growth hormone receptor antibody [MM0321-3C59] (ab89400)

Overview

  • Product nameAnti-Growth hormone receptor antibody [MM0321-3C59]
    See all Growth hormone receptor primary antibodies
  • Description
    Mouse monoclonal [MM0321-3C59] to Growth hormone receptor
  • Tested applicationsSuitable for: WB, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human Growth hormone receptor extracellular domain.

  • General notesReconstituted antibody should be aliquoted and can be stored frozen at < -20 for at least six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Properties

Applications

Our Abpromise guarantee covers the use of ab89400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 72 kDa.
Neutralising Use at an assay dependent dilution.

Target

  • FunctionReceptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway.
    The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
    Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling.
  • Tissue specificityExpressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 is expressed in lung, stomach and muscle. Low levels in liver.
  • Involvement in diseaseDefects in GHR are a cause of Laron syndrome (LARS) [MIM:262500]. A severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone.
    Defects in GHR may be a cause of idiopathic short stature autosomal (ISSA) [MIM:604271]. Short stature is defined by a subnormal rate of growth.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 1 fibronectin type-III domain.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
    The extracellular domain is the ligand-binding domain representing the growth hormone-binding protein (GHBP).
    The ubiquitination-dependent endocytosis motif (UbE) is required for recruitment of the ubiquitin conjugation system on to the receptor and for its internalization.
  • Post-translational
    modifications
    The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17.
    On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2.
    On ligand binding, ubiquitinated on lysine residues in the cytoplasmic domain. This ubiquitination is not sufficient for GHR internalization.
  • Cellular localizationSecreted; Cell membrane. On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway and Cell membrane. Remains fixed to the cell membrane and is not internalized.
  • Information by UniProt
  • Database links
  • Alternative names
    • GH receptor antibody
    • GH-binding protein antibody
    • GHBP antibody
    • GHBP, included antibody
    • GHR antibody
    • GHR_HUMAN antibody
    • Growth hormone binding protein antibody
    • Growth hormone receptor antibody
    • Growth hormone receptor precursor antibody
    • Growth hormone-binding protein antibody
    • Growth hormone-binding protein, included antibody
    • Increased responsiveness to growth hormone, included antibody
    • Serum binding protein antibody
    • Serum-binding protein antibody
    • Somatotropin receptor antibody
    see all

References for Anti-Growth hormone receptor antibody [MM0321-3C59] (ab89400)

ab89400 has not yet been referenced specifically in any publications.

Product Wall

While an antibody created from an immunogen with 73% homology with a target of interest may have reactivity, Abcam does not recommend use of a product which has less than 85% homology.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"