• Product name
    Anti-Grp75 antibody [JG1]
    See all Grp75 primary antibodies
  • Description
    Mouse monoclonal [JG1] to Grp75
  • Host species
  • Tested applications
    Suitable for: WB, IP, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Hamster, Dog, Human, Non human primates
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Mouse Grp75 aa 661-679.


    Database link: P38647

  • General notes





Our Abpromise guarantee covers the use of ab2799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IP Use at an assay dependent concentration.
ICC/IF 1/50 - 1/200.

Staining of mtHSP 70 in DAP.3 cells results in a worm-like staining pattern, consistent with mitochondrial localization.

IHC-P Use a concentration of 1 µg/ml.
Flow Cyt 1/100.

ab91537 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.



  • Function
    Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
  • Sequence similarities
    Belongs to the heat shock protein 70 family.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • 75 kDa glucose regulated protein antibody
    • 75 kDa glucose-regulated protein antibody
    • CSA antibody
    • Glucose Regulated Protein antibody
    • Grp 75 antibody
    • GRP-75 antibody
    • GRP75 antibody
    • GRP75_HUMAN antibody
    • Heat shock 70 kDa protein 9 antibody
    • Heat shock 70kD protein 9 antibody
    • heat shock 70kDa protein 9 antibody
    • Heat shock 70kDa protein 9B antibody
    • Heat shock protein 74 kDa A antibody
    • Heat shock protein A antibody
    • Heat shock protein cognate 74 antibody
    • Hsc74 antibody
    • Hsp74 antibody
    • Hsp74a antibody
    • HSPA9 antibody
    • Hspa9a antibody
    • HSPA9B antibody
    • MGC4500 antibody
    • mitochondrial antibody
    • Mortalin 2 antibody
    • Mortalin antibody
    • Mortalin perinuclear antibody
    • Mortalin2 antibody
    • MOT 2 antibody
    • MOT antibody
    • MOT2 antibody
    • Mthsp70 antibody
    • p66 mortalin antibody
    • P66 MOT antibody
    • PBP74 antibody
    • Peptide binding protein 74 antibody
    • Peptide-binding protein 74 antibody
    • Stress 70 protein mitochondrial antibody
    • Stress 70 protein mitochondrial precursor antibody
    • Stress-70 protein antibody
    see all


  • Immunocytochemistry/Immunofluorescence analysis of Grp75 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA for 15 minutes at room temperature. Cells were incubated with (left panel) or without (right panel) ab2799 (1:50) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody (1:400) for 30 minutes at room temperature. F-Actin (red) was stained with Dylight 554 phalloidin, and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized human testis tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a Mouse Monoclonal Antibody recognizing Grp75 (ab2799) or without primary antibody (negative control) overnight at 4�C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • ICC/IF image of ab2799 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2799, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Grp75 antibody [JG1] (ab2799) at 1/750 dilution + Mouse brain tissue lysates at 10 µg

    HRP conjugated donkey polyclonal to mouse IgG3 at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 74 kDa
    Observed band size: 74 kDa

    Exposure time: 3 minutes

    Primary incubated for 1 hour 30 minutes at 24°C.

  • Anti-Grp75 antibody [JG1] (ab2799) at 1/750 dilution
    HRP conjugated donkey anti mouse IgG3 at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 74 kDa
    Observed band size: 74 kDa

    Exposure time: 5 minutes

    See Abreview

  • ab2799 (1µg/ml) staining GPR75 in human frontal cerebral cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is strong mitochondrial staining.
    Insert depicts negative control (no primary antibody).
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Overlay histogram showing HepG2 cells stained with ab2799 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2799, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunocytochemistry/Immunofluorescence analysis of human fibroblasts labelling Grp75 with ab2799.


This product has been referenced in:
  • Burger K  et al. Nuclear phosphorylated Dicer processes double-stranded RNA in response to DNA damage. J Cell Biol 216:2373-2389 (2017). Human . Read more (PubMed: 28642363) »
  • Lin H  et al. Early cerebellar deficits in mitochondrial biogenesis and respiratory chain complexes in the KIKO mouse model of Friedreich ataxia. Dis Model Mech 10:1343-1352 (2017). Read more (PubMed: 29125827) »

See all 18 Publications for this product

Customer reviews and Q&As

Merci de nous avoir contactés. L'anticorps ab2799 est dirigé contre la protéine Grp75, référence SwissProt http://www.uniprot.org/uniprot/P38646 La liste des anti-Hsp70 (SwissProt http://www.uniprot.org/uniprot/P08107 ) est : http://ww...

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Thank you for your enquiry. This antibody has been tested in Western blotting against equal loadings of purified wild type survivin, 2B and DEx3. When used at 1/500, it detects a single clean band of the appropriate size for 2B, but no band is det...

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Thank you for your enquiry and your patience. We do not sell a positive control for this antibody. We have not succeeded in detecting endogenous Survivin 2B using this antibody (Survivin 2B is expressed at very low levels). This antibody has bee...

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Thank you for your enquiry regarding the protein concentration of ab2799. To answer your question, the concentration has been determined as : 2.9 mg/mL. If you need addditional information, please contact us again and we will gladly assist you.


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