Anti-GRP78 BiP antibody [10C3] (DyLight® 488) (ab115638)

Overview

  • Product name
    Anti-GRP78 BiP antibody [10C3] (DyLight® 488)
    See all GRP78 BiP primary antibodies
  • Description
    Mouse monoclonal [10C3] to GRP78 BiP (DyLight® 488)
  • Conjugation
    DyLight® 488. Ex: 493nm, Em: 518nm
  • Specificity
    Recognizes proteins containing the KDEL sequence.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Bird, Plants, Mammal
  • Immunogen

    Synthetic peptide corresponding to Rat GRP78 BiP aa 649-654.
    Sequence:

    SEKDEL

  • Positive control
    • Jurkat cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    10C3
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20.

Target

  • Function
    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
  • Involvement in disease
    Autoantigen in rheumatoid arthritis.
  • Sequence similarities
    Belongs to the heat shock protein 70 family.
  • Cellular localization
    Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 78 kDa glucose regulated protein antibody
    • 78 kDa glucose-regulated protein antibody
    • AL022860 antibody
    • AU019543 antibody
    • BIP antibody
    • D2Wsu141e antibody
    • D2Wsu17e antibody
    • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
    • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
    • Epididymis secretory sperm binding protein Li 89n antibody
    • FLJ26106 antibody
    • Glucose Regulated Protein 78kDa antibody
    • GRP 78 antibody
    • GRP-78 antibody
    • GRP78 antibody
    • GRP78_HUMAN antibody
    • Heat shock 70 kDa protein 5 antibody
    • Heat Shock 70kDa Protein 5 antibody
    • Heat shock protein family A (Hsp70) member 5 antibody
    • HEL S 89n antibody
    • Hsce70 antibody
    • HSPA 5 antibody
    • HSPA5 antibody
    • Immunoglobulin Heavy Chain Binding Protein antibody
    • Immunoglobulin heavy chain-binding protein antibody
    • mBiP antibody
    • MIF2 antibody
    • Sez7 antibody
    see all

Images

  • ab115638 at 10µg/ml staining GRP78 in 506 Jurkat cells by Flow cytometry (shown in green).
    Grey: unstained;Yellow: isotype control.

References

ab115638 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us. This antibody has not been tested without permeabilization. Due to its intracellular localization, the laboratory skeptical that it would work this way.

I hope this information is helpful to you. Please do not hesi...

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Thank you for contacting us regarding the flow cytometry protocol for ab115638.

I do wish to apologize for the delay in getting this information to you. The protocol used by the lab is attached.

I hope this information is hel...

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Thank you for contacting us. Unofrtunately we do not have the Dylight 488 isotype control available in our catalog.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information...

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Thank you for contacting Abcam and for your interest in ab115638.


As we discussed, I have updated the datasheet to reflect that human is a tested species guaranteed by our Abpromise. I apologize for the discrepancy and for any confusion t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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