• Product nameAnti-GRP78 BiP antibody [76-E6]
    See all GRP78 BiP primary antibodies
  • Description
    Rat monoclonal [76-E6] to GRP78 BiP
  • SpecificityThis antibody has been shown to be useful in studies of immunoglobulin assembly and secretion.
  • Tested applicationsSuitable for: Functional Studies, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Hamster, Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • EpitopeThis antibody recognizes a conserved epitope localized within the region of amino acids 497 to 581 of BiP.
  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human breast fibroadenoma.
  • General notes


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer100 mM borate buffered saline, pH 8.0.
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number76-E6
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab25192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution. Immunoglobulin assembly and secretion.
IHC-P Use a concentration of 10 µg/ml.
WB Use at an assay dependent dilution. Predicted molecular weight: 72 kDa.
ELISA Use at an assay dependent dilution.


  • FunctionProbably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
  • Involvement in diseaseAutoantigen in rheumatoid arthritis.
  • Sequence similaritiesBelongs to the heat shock protein 70 family.
  • Cellular localizationEndoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 78 kDa glucose regulated protein antibody
    • 78 kDa glucose-regulated protein antibody
    • AL022860 antibody
    • AU019543 antibody
    • BIP antibody
    • D2Wsu141e antibody
    • D2Wsu17e antibody
    • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
    • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
    • Epididymis secretory sperm binding protein Li 89n antibody
    • FLJ26106 antibody
    • Glucose Regulated Protein 78kDa antibody
    • GRP 78 antibody
    • GRP-78 antibody
    • GRP78 antibody
    • GRP78_HUMAN antibody
    • Heat shock 70 kDa protein 5 antibody
    • Heat Shock 70kDa Protein 5 antibody
    • Heat shock protein family A (Hsp70) member 5 antibody
    • HEL S 89n antibody
    • Hsce70 antibody
    • HSPA 5 antibody
    • HSPA5 antibody
    • Immunoglobulin Heavy Chain Binding Protein antibody
    • Immunoglobulin heavy chain-binding protein antibody
    • mBiP antibody
    • MIF2 antibody
    • Sez7 antibody
    see all

Anti-GRP78 BiP antibody [76-E6] images

  • IHC image of GRP78 BiP staining in XHuman breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25192, 10µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Predicted band size : 72 kDa

    Total cell lysates from Ag8.653 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Anti-GRP78 BiP antibody [76-E6] ab25192 (2 µg/mL). Proteins were visualized using Goat Anti-Rat Ig, Mouse ads-HRP secondary antibody and chemiluminescent detection.

References for Anti-GRP78 BiP antibody [76-E6] (ab25192)

This product has been referenced in:
  • Fausther-Bovendo H  et al. HIV gp41 engages gC1qR on CD4+ T cells to induce the expression of an NK ligand through the PIP3/H2O2 pathway. PLoS Pathog 6:e1000975 (2010). ICC/IF ; Human . Read more (PubMed: 20617170) »

See 1 Publication for this product

Product Wall

Application ELISA
Sample Human Recombinant protein (Blood Plasma)
Specification Blood Plasma
Type Sandwich (Capture)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 16 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
Sample Mouse Tissue sections (brain hemisphere coronal sections)
Specification brain hemisphere coronal sections
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2 · Temperature: 25°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 16 2013