The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Immunoglobulin assembly and secretion.
Use a concentration of 10 µg/ml.
Use at an assay dependent dilution. Predicted molecular weight: 72 kDa.
Use at an assay dependent dilution.
FunctionProbably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Involvement in diseaseAutoantigen in rheumatoid arthritis.
Sequence similaritiesBelongs to the heat shock protein 70 family.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
IHC image of GRP78 BiP staining in XHuman breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25192, 10µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-GRP78 BiP antibody [76-E6] (ab25192)
Predicted band size : 72 kDa
Total cell lysates from Ag8.653 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Anti-GRP78 BiP antibody [76-E6] ab25192 (2 µg/mL). Proteins were visualized using Goat Anti-Rat Ig, Mouse ads-HRP secondary antibody and chemiluminescent detection.
References for Anti-GRP78 BiP antibody [76-E6] (ab25192)
This product has been referenced in:
Zhang S et al. Novel Role of ER Stress and Autophagy in Microcystin-LR Induced Apoptosis in Chinese Hamster Ovary Cells. Front Physiol7:527 (2016).
Read more (PubMed: 27877136) »
Fausther-Bovendo H et al. HIV gp41 engages gC1qR on CD4+ T cells to induce the expression of an NK ligand through the PIP3/H2O2 pathway. PLoS Pathog6:e1000975 (2010).
Read more (PubMed: 20617170) »