The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
FuncS: Use at an assay dependent dilution.
Note: ab25695 can be used for studies of immunoglobulin assembly and secretion.
WB: Use 10µg for 106 cells.Predicted molecular weight: 72 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionProbably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Involvement in diseaseAutoantigen in rheumatoid arthritis.
Sequence similaritiesBelongs to the heat shock protein 70 family.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.