Anti-GRP78 BiP antibody [76-E6] (Biotin) (ab25695)


  • Product nameAnti-GRP78 BiP antibody [76-E6] (Biotin)
    See all GRP78 BiP primary antibodies
  • Description
    Rat monoclonal [76-E6] to GRP78 BiP (Biotin)
  • ConjugationBiotin
  • Specificityab25695 recognises GRP78 BiP.
  • Tested applicationsSuitable for: WB, ELISA, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse, Hamster
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Epitopeab25695 rects with a conserved epitope located within the region of amino acids 497 to 581 of BiP.
  • Positive control
    • cell lysate from Ag8.653


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number76-E6
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab25695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
  • Application notesELISA: Use at an assay dependent dilution.
    FuncS: Use at an assay dependent dilution.
    Note: ab25695 can be used for studies of immunoglobulin assembly and secretion.
    WB: Use 10µg for 106 cells.Predicted molecular weight: 72 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionProbably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
    • Involvement in diseaseAutoantigen in rheumatoid arthritis.
    • Sequence similaritiesBelongs to the heat shock protein 70 family.
    • Cellular localizationEndoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • 78 kDa glucose regulated protein antibody
      • 78 kDa glucose-regulated protein antibody
      • AL022860 antibody
      • AU019543 antibody
      • BIP antibody
      • D2Wsu141e antibody
      • D2Wsu17e antibody
      • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
      • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
      • Epididymis secretory sperm binding protein Li 89n antibody
      • FLJ26106 antibody
      • Glucose Regulated Protein 78kDa antibody
      • GRP 78 antibody
      • GRP-78 antibody
      • GRP78 antibody
      • GRP78_HUMAN antibody
      • Heat shock 70 kDa protein 5 antibody
      • Heat Shock 70kDa Protein 5 antibody
      • Heat shock protein family A (Hsp70) member 5 antibody
      • HEL S 89n antibody
      • Hsce70 antibody
      • HSPA 5 antibody
      • HSPA5 antibody
      • Immunoglobulin Heavy Chain Binding Protein antibody
      • Immunoglobulin heavy chain-binding protein antibody
      • mBiP antibody
      • MIF2 antibody
      • Sez7 antibody
      see all

    Anti-GRP78 BiP antibody [76-E6] (Biotin) images

    • All lanes : Anti-GRP78 BiP antibody [76-E6] (Biotin) (ab25695) at 10 µg/ml

      Lane 1 : Recombinant hamster BiP mutant HCB1-.
      Lane 2 : full length recombinant hamster BiP.
      Lane 3 : total cell lysate from Ag8.653, a mouse myeloma cell line.

      Developed using the ECL technique

      Predicted band size : 72 kDa
      Observed band size : 72,123,150 kDa (why is the actual band size different from the predicted?)

    References for Anti-GRP78 BiP antibody [76-E6] (Biotin) (ab25695)

    ab25695 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab25695.
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