Anti-GRP78 BiP antibody [9E4-2A7-H6] - C-terminal (ab181499)

Overview

  • Product name
    Anti-GRP78 BiP antibody [9E4-2A7-H6] - C-terminal
    See all GRP78 BiP primary antibodies
  • Description
    Mouse monoclonal [9E4-2A7-H6] to GRP78 BiP - C-terminal
  • Host species
    Mouse
  • Specificity
    ab181499 does not cross-react with related proteins.
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human GRP78 BiP (C terminal). Expressed in E.coli.
    Database link: P11021

  • Positive control
    • HeLa, C6, Lncap and MDA-MB-468 lysates; HeLa cells.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab21685 as a replacement.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    9E4-2A7-H6
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 72 kDa.
ICC/IF 1/50.

Target

  • Function
    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
  • Involvement in disease
    Autoantigen in rheumatoid arthritis.
  • Sequence similarities
    Belongs to the heat shock protein 70 family.
  • Cellular localization
    Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 78 kDa glucose regulated protein antibody
    • 78 kDa glucose-regulated protein antibody
    • AL022860 antibody
    • AU019543 antibody
    • BIP antibody
    • D2Wsu141e antibody
    • D2Wsu17e antibody
    • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
    • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
    • Epididymis secretory sperm binding protein Li 89n antibody
    • FLJ26106 antibody
    • Glucose Regulated Protein 78kDa antibody
    • GRP 78 antibody
    • GRP-78 antibody
    • GRP78 antibody
    • GRP78_HUMAN antibody
    • Heat shock 70 kDa protein 5 antibody
    • Heat Shock 70kDa Protein 5 antibody
    • Heat shock protein family A (Hsp70) member 5 antibody
    • HEL S 89n antibody
    • Hsce70 antibody
    • HSPA 5 antibody
    • HSPA5 antibody
    • Immunoglobulin Heavy Chain Binding Protein antibody
    • Immunoglobulin heavy chain-binding protein antibody
    • mBiP antibody
    • MIF2 antibody
    • Sez7 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of colorectal cancer labeling GRP78 with ab181499 at 1/100. Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

  • Immunofluorescent anaysis of HeLa cells (4% Paraformaldehyde-fixed) labeling GRP78 with ab181499 at 1/50 dilution.

  • All lanes : Anti-GRP78 BiP antibody [9E4-2A7-H6] - C-terminal (ab181499) at 1/1000 dilution

    Lane 1 : LnCap lysate
    Lane 2 : C6 lysate
    Lane 3 : MDA-MB-468 lysate
    Lane 4 : HeLa lysate

    Predicted band size: 72 kDa

References

ab181499 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181499.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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