1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Involvement in disease
Autoantigen in rheumatoid arthritis.
Belongs to the heat shock protein 70 family.
Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ab103336 at 1/50 dilution staining GRP78 BiP in formalin-fixed and paraffin-embedded Human lung carcinoma by Immunohistochemistry, followed by peroxidase conjugation of the secondary antibody and DAB detection.
Booth L et al. PDE5 inhibitors enhance the lethality of pemetrexed through inhibition of multiple chaperone proteins and via the actions of cyclic GMP and nitric oxide. Oncotarget8:1449-1468 (2017).
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