Recombinant
RabMAb

Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)

Submit an Abreview Q&A (6)Specific References (9)

Overview

  • Product name
    Anti-GRP78 BiP antibody [EPR4040(2)]
    See all GRP78 BiP primary antibodies
  • Description
    Rabbit monoclonal [EPR4040(2)] to GRP78 BiP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human GRP78 BiP.

  • Positive control
    • HeLa, LnCaP, ECV-304, C6, NIH/3T3, PC-12, and RAW264.7 and HepG2 cell lysates. Human brain and testis tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR4040(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa).Can be blocked with GRP78 BiP peptide (ab176143).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function
      Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
    • Involvement in disease
      Autoantigen in rheumatoid arthritis.
    • Sequence similarities
      Belongs to the heat shock protein 70 family.
    • Cellular localization
      Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • 78 kDa glucose regulated protein antibody
      • 78 kDa glucose-regulated protein antibody
      • AL022860 antibody
      • AU019543 antibody
      • BIP antibody
      • D2Wsu141e antibody
      • D2Wsu17e antibody
      • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
      • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
      • Epididymis secretory sperm binding protein Li 89n antibody
      • FLJ26106 antibody
      • Glucose Regulated Protein 78kDa antibody
      • GRP 78 antibody
      • GRP-78 antibody
      • GRP78 antibody
      • GRP78_HUMAN antibody
      • Heat shock 70 kDa protein 5 antibody
      • Heat Shock 70kDa Protein 5 antibody
      • Heat shock protein family A (Hsp70) member 5 antibody
      • HEL S 89n antibody
      • Hsce70 antibody
      • HSPA 5 antibody
      • HSPA5 antibody
      • Immunoglobulin Heavy Chain Binding Protein antibody
      • Immunoglobulin heavy chain-binding protein antibody
      • mBiP antibody
      • MIF2 antibody
      • Sez7 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP78 BiP antibody [EPR4040(2)] (ab108613)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP78 BiP antibody [EPR4040(2)] (ab108613)
      Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108613 at 1/250
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP78 BiP antibody [EPR4040(2)] (ab108613)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP78 BiP antibody [EPR4040(2)] (ab108613)
      Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab108613 at 1/250
    • Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)
      Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)
      All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/5000 dilution (purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ug/ml
      tunicamycin for 12 hours whole cell lysates
      Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
      Lane 4 : MCF-7 (Human breast adenocarcinoma epithelial cell) treated with 10ug/ml
      tunicamycin for 48 hours whole cell lysates
      Lane 5 : MEF (Mouse embryonic fibroblast) whole cell lysates
      Lane 6 : MEF (Mouse embryonic fibroblast) treated with 10ug/ml tunicamycin for 48 hours whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 72 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

      Tunicamycin induces the expression of GRP78 BiP reported by PMID 22859938

      Exposure time:
      Lanes 1 and 2: 3 seconds 
      Lanes 3 and 4: 30 seconds
      Lanes 5 and 6: 5 seconds

    • Western blot - GRP78 BiP antibody [EPR4040(2)] (ab108613)
      Western blot - GRP78 BiP antibody [EPR4040(2)] (ab108613)
      All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : LnCaP cell lysate
      Lane 3 : ECV-304 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 72 kDa
      Observed band size: 78 kDa (why is the actual band size different from the predicted?)

    • Western blot - GRP78 BiP antibody [EPR4040(2)] (ab108613)
      Western blot - GRP78 BiP antibody [EPR4040(2)] (ab108613)
      All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : NIH/3T3 cell lysate
      Lane 3 : PC-12 cell lysate
      Lane 4 : RAW264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 72 kDa
      Observed band size: 78 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Oh JH  et al. Negative regulation of ERK1/2 by PI3K is required for the protective effects of Pyropia yezoensis peptide against perfluorooctane sulfonate-induced endoplasmic reticulum stress. Mol Med Rep 15:2583-2587 (2017). WB . Read more (PubMed: 28260037) »
    • Shen J  et al. GRP78 haploinsufficiency suppresses acinar-to-ductal metaplasia, signaling, and mutantKras-driven pancreatic tumorigenesis in mice. Proc Natl Acad Sci U S A 114:E4020-E4029 (2017). Read more (PubMed: 28461470) »

    See all 9 Publications for this product

    Publishing research using ab108613? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    6 reviews or Q&A

    10
    Votes: Highest First

    What is the IHC-P protocol for ab108613?

    Thank you for contacting us. Below is the protocol used to validate ab108613 in IHC-P. Please let me know if you need any additional information or assistance.

    1. Solutions and reagents
    1.1. Xylene
    1.2. Ethanol, anhydrous denatured...

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    Thanks for helping out with this inquiry. I attached an annotated power point of the data and the page from my lab notebook that contains the blotting map. If you need anything else from me, let me know.



    "We only saw a faint band at 50 kDa using mouse and rat heart, which looks non-specific on our blots. I have not found the identity of this band in the literature. The blocking reagent used was 5% milk. We used our standard WB protocol (...

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    Thank you for your reply. I am so sorry to tell you that we cantest the GRP94 protein which has the similar function and position withGRP78 by western blotting.We extracted the GRP94 protein from SD brain
    tissue using our lysis buffer.The att...

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    Thank you for your reply.
    If you were able to detect a different ER protein using the same samples then it doesn't seem like it was a problem with the sample preparation.
    I would like to offer you a free of charge replacement of ab108613 (alt...

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    We can not get the positive result based on the protocol. Thanks!

    Thank you for taking time to send us your protocol. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring prod...

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    Phone call to confirm to replace ab2902 with ab108613 and to accept testing discount for ab108613 in ICC/IF.


    Merci de votre appel. Je suis ravie que vous souhaitiez participer a notre offre et tester ab108613 en immunofluorescence sur des cellules (ICC/IF). Le code de réduction correspondant est xx. Ce code de réduction est utilisable sous 120 jours et p...

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    Our customer makes a request about Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613). She has ordered the ab108613 recently. Would you please offer the COC for her?

    Thank you for contacting us, please find the requested CoC attached. Please let me know if you need any other information!

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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