Synthetic peptide corresponding to residues in Human GRP78 BiP.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab108613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa).Can be blocked with GRP78 BiP peptide (ab176143).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)|
Blocking and diluting buffer: 5% NFDM/TBST.
Tunicamycin induces the expression of GRP78 BiP reported by PMID 22859938
Lanes 1 and 2: 3 seconds
Lanes 3 and 4: 30 seconds
Lanes 5 and 6: 5 seconds
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"