Recombinant
Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)
- Datasheet
- Specific References (4)
- Protocols
Overview
- Product nameAnti-GRP78 BiP antibody [EPR4040(2)]
See all GRP78 BiP primary antibodies - DescriptionRabbit monoclonal [EPR4040(2)] to GRP78 BiP
- Tested applicationsSuitable for:
WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP - Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide corresponding to residues in Human GRP78 BiP.
- Positive control
- HeLa, LnCaP, ECV-304, C6, NIH/3T3, PC-12, and RAW264.7 and HepG2 cell lysates. Human brain and testis tissue.
- General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- ClonalityMonoclonal
- Clone numberEPR4040(2)
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
- Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab108613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa).Can be blocked with GRP78 BiP peptide (ab176143). | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) |
Target
- FunctionProbably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
- Involvement in diseaseAutoantigen in rheumatoid arthritis.
- Sequence similaritiesBelongs to the heat shock protein 70 family.
- Cellular localizationEndoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 3309 Human
- Entrez Gene: 14828 Mouse
- Entrez Gene: 25617 Rat
- Omim: 138120 Human
- SwissProt: P11021 Human
- SwissProt: P20029 Mouse
- SwissProt: P06761 Rat
- Unigene: 743241 Human
see all -
Alternative names
- 78 kDa glucose regulated protein antibody
- 78 kDa glucose-regulated protein antibody
- AL022860 antibody
see all
Anti-GRP78 BiP antibody [EPR4040(2)] images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP78 BiP antibody [EPR4040(2)] (ab108613)](http://a.abcam.com/ps/products/108/ab108613/Images/GRP78-BiP-Primary-antibodies-ab108613-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP78 BiP antibody [EPR4040(2)] (ab108613)Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108613 at 1/250 -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP78 BiP antibody [EPR4040(2)] (ab108613)](http://a.abcam.com/ps/products/108/ab108613/Images/GRP78-BiP-Primary-antibodies-ab108613-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP78 BiP antibody [EPR4040(2)] (ab108613)Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab108613 at 1/250 -
![Western blot - GRP78 BiP antibody [EPR4040(2)] (ab108613)](http://a.abcam.com/ps/products/108/ab108613/Images/GRP78-BiP-Primary-antibodies-ab108613-1.jpg)
Western blot - GRP78 BiP antibody [EPR4040(2)] (ab108613)All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : LnCaP cell lysate
Lane 3 : ECV-304 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 72 kDa
Observed band size : 78 kDa (why is the actual band size different from the predicted?) -
![Western blot - GRP78 BiP antibody [EPR4040(2)] (ab108613)](http://a.abcam.com/ps/products/108/ab108613/Images/GRP78-BiP-Primary-antibodies-ab108613-2.jpg)
Western blot - GRP78 BiP antibody [EPR4040(2)] (ab108613)All lanes : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : NIH/3T3 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : RAW264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 72 kDa
Observed band size : 78 kDa (why is the actual band size different from the predicted?)
References for Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613)
This product has been referenced in:
- Svennerholm K et al. DNA Content in Extracellular Vesicles Isolated from Porcine Coronary Venous Blood Directly after Myocardial Ischemic Preconditioning. PLoS One 11:e0159105 (2016). WB . Read more (PubMed: 27434143) »
- Russ DW et al. Muscle-specificity of age-related changes in markers of autophagy and sphingolipid metabolism. Biogerontology 16:747-59 (2015). WB ; Rat . Read more (PubMed: 26296420) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"