Recombinant Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4041(2)] to GRP78 BiP
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GRP78 BiP antibody [EPR4041(2)]
See all GRP78 BiP primary antibodies -
Description
Rabbit monoclonal [EPR4041(2)] to GRP78 BiP -
Host species
Rabbit -
Specificity
Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, NIH/3T3, LnCaP, MCF7, 293T, U-87 MG, THP-1, MDA-MB-231 and HepG2 whole cell lysate (ab7900); Human liver lysates, Mouse heart, lung, liver and testis lysates; Rat liver lysates. Huvec and RAW 264.7 cells treated with tunicamycin. IHC-P: Human breast carcinoma Tissue Flow Cyt (intra): HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4041(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/200.
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images for recommended treatment conditions and positive controls. |
IHC-P | (1) |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified, use 1/50 - 1/100. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Notes |
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Flow Cyt (Intra)
1/200. |
WB
1/1000 - 1/10000. Predicted molecular weight: 72 kDa. Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images for recommended treatment conditions and positive controls. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified, use 1/50 - 1/100. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Target
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Function
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. -
Involvement in disease
Autoantigen in rheumatoid arthritis. -
Sequence similarities
Belongs to the heat shock protein 70 family. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 3309 Human
- Entrez Gene: 14828 Mouse
- Entrez Gene: 25617 Rat
- Omim: 138120 Human
- SwissProt: P11021 Human
- SwissProt: P20029 Mouse
- SwissProt: P06761 Rat
- Unigene: 743241 Human
see all -
Alternative names
- 78 kDa glucose regulated protein antibody
- 78 kDa glucose-regulated protein antibody
- AL022860 antibody
see all
Images
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All lanes : Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution (purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ug/ml
tunicamycin for 12 hours whole cell lysates
Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : MCF-7 (Human breast adenocarcinoma epithelial cell) treated with 10ug/ml
tunicamycin for 48 hours whole cell lysates
Lane 5 : MEF (Mouse embryonic fibroblast) whole cell lysates
Lane 6 : MEF (Mouse embryonic fibroblast) treated with 10ug/ml tunicamycin for 48 hours whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 72 kDaBlocking and diluting buffer: 5% NFDM/TBST.
Tunicamycin induces the expression of GRP78 BiP reported by PMID 22859938Exposure time:
Lanes 1 and 2: 20 seconds
Lanes 3 and 4: 30 seconds
Lanes 5 and 6: 5 seconds -
Immunohistochemical staining of paraffin embedded human hepatocellular carcinoma with purified ab108615 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Unpurified ab108615 at 1/50 dilution staining GRP78 BiP in Human breast carcinoma tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 3 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lane 5 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
Lane 6 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 8 : Human uterus lysates
Lane 9 : Human liver lysates
Lane 10 : Mouse heart lysates
Lane 11 : Mouse lung lysates
Lane 12 : Mouse testis lysates
Lane 13 : Mouse liver lysates
Lane 14 : Rat liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa treated with 2.5 µg/ml tunicamycin for 24h whole cell lysates
Lane 3 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates
Lane 4 : HUVEC treated with 10 µg/ml tunicamycin for 48h whole cell lysates
Lane 5 : Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 6 : Raw 264.7 treated with 5 µg/ml tunicamycin for 18h whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysates
Lane 2 : NIH/3T3 cell lysates
Lane 3 : LnCaP cell lysates
Lane 4 : HepG2 cell lysates
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 72 kDa -
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GRP78 BiP with purified ab108615 at 1/200 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution (purified) + Raw264.7 cell lysate at 20 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/5000 dilution (purified) + HepG2 at 20 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution (purified) + LnCaP at 20 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (60)
ab108615 has been referenced in 60 publications.
- Wang F & Han L Upregulation of serum and glucocorticoid-regulated kinase 1 (SGK1) ameliorates doxorubicin-induced cardiotoxic injury, apoptosis, inflammation and oxidative stress by suppressing glucose regulated protein 78 (GRP78)-mediated endoplasmic reticulum stress. Bioengineered 13:844-855 (2022). PubMed: 34898378
- Yao Y et al. circ_014260/miR-384/THBS1 aggravates spinal cord injury in rats by promoting neuronal apoptosis and endoplasmic reticulum stress. Am J Transl Res 14:518-533 (2022). PubMed: 35173872
- Cai J et al. SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β‑catenin/TCF/LEF signaling pathway. Mol Med Rep 25:N/A (2022). PubMed: 35419615
- Wu L et al. A natural allele of OsMS1 responds to temperature changes and confers thermosensitive genic male sterility. Nat Commun 13:2055 (2022). PubMed: 35440626
- Zhang H et al. Rutaecarpine ameliorates lipopolysaccharide-induced BEAS-2B cell injury through inhibition of endoplasmic reticulum stress via activation of the AMPK/SIRT1 signaling pathway. Exp Ther Med 23:373 (2022). PubMed: 35495603