Recombinant
RabMAb

Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

Overview

  • Product name
    Anti-GRP78 BiP antibody [EPR4041(2)]
    See all GRP78 BiP primary antibodies
  • Description
    Rabbit monoclonal [EPR4041(2)] to GRP78 BiP
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human GRP78 BiP.

  • Positive control
    • WB: HeLa, NIH/3T3, LnCaP, and HepG2 cell lysates IHC-P: Human breast carcinoma tissue FC: HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR4041(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200.
ICC/IF 1/200.

For unpurified, use 1/50 - 1/100.

WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.Can be blocked with GRP78 BiP peptide (ab176142).
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified, use 1/50 - 1/100.

Target

  • Function
    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
  • Involvement in disease
    Autoantigen in rheumatoid arthritis.
  • Sequence similarities
    Belongs to the heat shock protein 70 family.
  • Cellular localization
    Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 78 kDa glucose regulated protein antibody
    • 78 kDa glucose-regulated protein antibody
    • AL022860 antibody
    • AU019543 antibody
    • BIP antibody
    • D2Wsu141e antibody
    • D2Wsu17e antibody
    • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
    • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
    • Epididymis secretory sperm binding protein Li 89n antibody
    • FLJ26106 antibody
    • Glucose Regulated Protein 78kDa antibody
    • GRP 78 antibody
    • GRP-78 antibody
    • GRP78 antibody
    • GRP78_HUMAN antibody
    • Heat shock 70 kDa protein 5 antibody
    • Heat Shock 70kDa Protein 5 antibody
    • Heat shock protein family A (Hsp70) member 5 antibody
    • HEL S 89n antibody
    • Hsce70 antibody
    • HSPA 5 antibody
    • HSPA5 antibody
    • Immunoglobulin Heavy Chain Binding Protein antibody
    • Immunoglobulin heavy chain-binding protein antibody
    • mBiP antibody
    • MIF2 antibody
    • Sez7 antibody
    see all

Anti-GRP78 BiP antibody [EPR4041(2)] images

  • Immunofluorescence staining of HeLa cells with purified ab108615 at a working dilution of 1/200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108615 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemical staining of paraffin embedded human hepatocellular carcinoma with purified ab108615 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • All lanes : Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysates
    Lane 2 : NIH/3T3 cell lysates
    Lane 3 : LnCaP cell lysates
    Lane 4 : HepG2 cell lysates

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 72 kDa
  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GRP78 BiP with purified ab108615 at 1/200 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Unpurified ab108615 at 1/50 dilution staining GRP78 BiP in Human breast carcinoma tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

  • Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution (purified) + Raw264.7 cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 72 kDa
    Observed band size : 78 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/5000 dilution (purified) + HepG2 at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 72 kDa
    Observed band size : 78 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution (purified) + LnCaP at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 72 kDa
    Observed band size : 78 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

References for Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

This product has been referenced in:
  • Kaliberov SA  et al. Retargeted adenoviruses for radiation-guided gene delivery. Cancer Gene Ther N/A:N/A (2016). Flow Cyt, ICC ; Human . Read more (PubMed: 27492853) »
  • Liu Z  et al. Adiponectin reduces ER stress-induced apoptosis through PPARa transcriptional regulation of ATF2 in mouse adipose. Cell Death Dis 7:e2487 (2016). WB ; Mouse . Read more (PubMed: 27882945) »

See all 2 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (HT-1080 Cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
2 µg
Specification
HT-1080 Cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

Submitted Feb 28 2017

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Sample
Mouse Cell (hepatocytes)
Specification
hepatocytes
Permeabilization
Yes - 0.1% Trition X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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