Overview

  • Product name
  • Description
    Rabbit polyclonal to GRP94
  • Tested applications
    Suitable for: ICC, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Hamster, Human, Monkey
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human) (Internal sequence).

Properties

Applications

Our Abpromise guarantee covers the use of ab18055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
ICC/IF
WB
  • Application notes

    ICC: 1/100 - 1/400.
    IF: 1/100 - 1/400.
    WB: 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Works in ICC/IF with methanol-fixed and 4% paraformaldehyde fixed cells.
  • Target

    • Function
      Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
    • Sequence similarities
      Belongs to the heat shock protein 90 family.
    • Cellular localization
      Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • 94 kDa glucose regulated protein antibody
      • 94 kDa glucose-regulated protein antibody
      • ECGP antibody
      • Endoplasmin antibody
      • Endothelial cell (HBMEC) glycoprotein antibody
      • ENPL_HUMAN antibody
      • Glucose regulated protein 94kDa antibody
      • gp96 antibody
      • gp96 homolog antibody
      • GRP 94 antibody
      • GRP-94 antibody
      • Heat shock protein 90 kDa beta member 1 antibody
      • heat shock protein 90kDa beta (Grp94), member 1 antibody
      • Heat shock protein, 90 kDa, beta, 1 antibody
      • HSP90B1 antibody
      • Stress inducible tumor rejection antigen GP96 antibody
      • TRA1 antibody
      • tumor rejection antigen (gp96) 1 antibody
      • Tumor rejection antigen 1 antibody
      • Tumor rejection antigen gp96 antibody
      • Tumor rejection antigen-1 (gp96) antibody
      see all

    Images

    • Methanol-fixed HEK293 cells stained at 1/200 with ab18055 followed by FITC conjugated secondary.



    • Predicted band size : 94 kDa


      Western blot of soluble fraction of monkey brain stained at 1/1000 with ab18055, visualised with HRP-conjugated secondary and ECL.

    References

    This product has been referenced in:
    • Whitmore LC  et al. A Common Genetic Variant in TLR1 Enhances Human Neutrophil Priming and Impacts Length of Intensive Care Stay in Pediatric Sepsis. J Immunol 196:1376-86 (2016). WB, IP ; Human . Read more (PubMed: 26729809) »
    • Kim N  et al. Interferon-inducible protein SCOTIN interferes with HCV replication through the autolysosomal degradation of NS5A. Nat Commun 7:10631 (2016). Read more (PubMed: 26868272) »

    See all 5 Publications for this product

    Customer reviews and Q&As

    Wir haben leider aktuell keinen validierten anti-Housekeeping Protein Antikörper der nur humane Proteine erkennt. Wir wissen, dass der ab3369 Click here (or use the following: http://www.abcam.com/index.html?datasheet=3369) gegen MRP1 nicht mit der Mau...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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