Anti-GRP94 antibody (ab3674)
Key features and details
- Rabbit polyclonal to GRP94
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GRP94 antibody
See all GRP94 primary antibodies -
Description
Rabbit polyclonal to GRP94 -
Host species
Rabbit -
Specificity
At a very high concentration (<1/100), this antibody may recognise PDI and calreticulin. We recommend using this antibody at a relatively high dilution. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Full length native protein (purified) corresponding to Dog GRP94 aa 1 to the C-terminus.
Database link: P41148 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/2000 - 1/5000. Detects a band of approximately 94 kDa. Can be used at dilutions up to 1/30,000.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF | (6) |
Use at an assay dependent concentration.
Used at a concentration of 1/100 for 30 min on human renal carcinoma cell (see Abreview). |
Notes |
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WB
1/2000 - 1/5000. Detects a band of approximately 94 kDa. Can be used at dilutions up to 1/30,000. |
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. Used at a concentration of 1/100 for 30 min on human renal carcinoma cell (see Abreview). |
Target
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Function
Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 7184 Human
- Entrez Gene: 22027 Mouse
- Omim: 191175 Human
- SwissProt: P14625 Human
- SwissProt: P08113 Mouse
- Unigene: 192374 Human
- Unigene: 87773 Mouse
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Alternative names
- 94 kDa glucose regulated protein antibody
- 94 kDa glucose-regulated protein antibody
- ECGP antibody
see all
Images
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Immunostaining of mouse embryonic fibroblasts with ab3674. (See Nakamura et al. 2001).
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Anti-GRP94 antibody (ab3674) at 1/2000 dilution + Cell lysates prepared from mouse fibroblasts
Western blot of mouse fibroblasts using ab3764 at 1/2000. -
ab3674 at a 1/100 dilution staining human renal cell carcinoma cells by ICC/IF. The cells were permeabilized with 0.2% triton X100 and then blocked with 1%BSA, 4% goat serum prior to incubation with the primary 1antibody for 30 min at 37 °C. Bound antibody was detected using an Alexa fluor ® 488 Goat anti-Rabbit IgG (H+L).
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Ab3674 (1/1000) staining GPR94 in human stomach using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of peptic and parietal cells of the gastric gland.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
All lanes : Anti-GRP94 antibody (ab3674) at 1/2000 dilution
Lane 1 : Whole cell lysate prepared from murine 3T3 cells
Lane 2 : Whole cell lysate prepared from murine 3T3 cells, treated with 2.5 ug/ml Tunicamycin 10 hours
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 94 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Protocols
Datasheets and documents
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Datasheet download
References (32)
ab3674 has been referenced in 32 publications.
- Corbacho-Alonso N et al. Prioritization of Candidate Biomarkers for Degenerative Aortic Stenosis through a Systems Biology-Based In-Silico Approach. J Pers Med 12:N/A (2022). PubMed: 35455758
- Barrera MD et al. Proteomic Discovery of VEEV E2-Host Partner Interactions Identifies GRP78 Inhibitor HA15 as a Potential Therapeutic for Alphavirus Infections. Pathogens 10:N/A (2021). PubMed: 33801554
- Duan YR et al. Exosomal microRNA-16-5p from human urine-derived stem cells ameliorates diabetic nephropathy through protection of podocyte. J Cell Mol Med 25:10798-10813 (2021). PubMed: 31568645
- Chen YW et al. Circ_0044516 Regulates miR-136/MAT2A Pathway to Facilitate Lung Cancer Development. J Immunol Res 2021:5510869 (2021). PubMed: 34258296
- Du Z et al. Extracellular vesicles-derived miR-150-5p secreted by adipose-derived mesenchymal stem cells inhibits CXCL1 expression to attenuate hepatic fibrosis. J Cell Mol Med 25:701-715 (2021). PubMed: 33342075