Overview

  • Product name
  • Description
    Goat polyclonal to GRP94
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Dog, Human, Cynomolgus monkey, Syrian hamster, Orangutan
  • Immunogen

    Synthetic peptide:

    C-KEGVKFDESEKTKE

    , corresponding to internal sequence amino acids 593-606 of Human GRP94

  • Positive control
    • 3T3 lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab52031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 5 µg/ml.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).
ELISA Use at an assay dependent dilution. Antibody detection limit dilution 1:32,000.

Target

  • Function
    Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • Sequence similarities
    Belongs to the heat shock protein 90 family.
  • Cellular localization
    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 94 kDa glucose regulated protein antibody
    • 94 kDa glucose-regulated protein antibody
    • ECGP antibody
    • Endoplasmin antibody
    • Endothelial cell (HBMEC) glycoprotein antibody
    • ENPL_HUMAN antibody
    • Glucose regulated protein 94kDa antibody
    • gp96 antibody
    • gp96 homolog antibody
    • GRP 94 antibody
    • GRP-94 antibody
    • Heat shock protein 90 kDa beta member 1 antibody
    • heat shock protein 90kDa beta (Grp94), member 1 antibody
    • Heat shock protein, 90 kDa, beta, 1 antibody
    • HSP90B1 antibody
    • Stress inducible tumor rejection antigen GP96 antibody
    • TRA1 antibody
    • tumor rejection antigen (gp96) 1 antibody
    • Tumor rejection antigen 1 antibody
    • Tumor rejection antigen gp96 antibody
    • Tumor rejection antigen-1 (gp96) antibody
    see all

Anti-GRP94 antibody images

  • Anti-GRP94 antibody (ab52031) at 0.03 µg/ml + 3T3 lysate (35µg protein in RIPA buffer).

    Predicted band size : 92 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • ab52031 staining GRP94 in human liver tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue section underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was used.

References for Anti-GRP94 antibody (ab52031)

ab52031 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Rat Tissue lysate - whole (liver and heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
liver and heart
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Mar 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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