Overview

  • Product name
  • Description
    Goat polyclonal to GRP94
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Dog, Human, Cynomolgus monkey, Syrian hamster, Orangutan
  • Immunogen

    Synthetic peptide:

    C-KEGVKFDESEKTKE

    , corresponding to internal sequence amino acids 593-606 of Human GRP94

  • Positive control
    • 3T3 lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab52031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 5 µg/ml.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).
ELISA Use at an assay dependent dilution. Antibody detection limit dilution 1:32,000.

Target

  • Function
    Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • Sequence similarities
    Belongs to the heat shock protein 90 family.
  • Cellular localization
    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 94 kDa glucose regulated protein antibody
    • 94 kDa glucose-regulated protein antibody
    • ECGP antibody
    • Endoplasmin antibody
    • Endothelial cell (HBMEC) glycoprotein antibody
    • ENPL_HUMAN antibody
    • Glucose regulated protein 94kDa antibody
    • gp96 antibody
    • gp96 homolog antibody
    • GRP 94 antibody
    • GRP-94 antibody
    • Heat shock protein 90 kDa beta member 1 antibody
    • heat shock protein 90kDa beta (Grp94), member 1 antibody
    • Heat shock protein, 90 kDa, beta, 1 antibody
    • HSP90B1 antibody
    • Stress inducible tumor rejection antigen GP96 antibody
    • TRA1 antibody
    • tumor rejection antigen (gp96) 1 antibody
    • Tumor rejection antigen 1 antibody
    • Tumor rejection antigen gp96 antibody
    • Tumor rejection antigen-1 (gp96) antibody
    see all

Images

  • Anti-GRP94 antibody (ab52031) at 0.03 µg/ml + 3T3 lysate (35µg protein in RIPA buffer).

    Predicted band size : 92 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • ab52031 staining GRP94 in human liver tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue section underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was used.

References

ab52031 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (liver and heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
liver and heart
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Mar 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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