Overview

  • Product nameAnti-GRP94 antibody
    See all GRP94 primary antibodies
  • Description
    Rabbit polyclonal to GRP94
  • SpecificityGRP94 antibody detects endogenous levels of total GRP94 protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • human breast carcinoma tissue; SKOV3 cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab53075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • FunctionMolecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • Sequence similaritiesBelongs to the heat shock protein 90 family.
  • Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 94 kDa glucose regulated protein antibody
    • 94 kDa glucose-regulated protein antibody
    • ECGP antibody
    • Endoplasmin antibody
    • Endothelial cell (HBMEC) glycoprotein antibody
    • ENPL_HUMAN antibody
    • Glucose regulated protein 94kDa antibody
    • gp96 antibody
    • gp96 homolog antibody
    • GRP 94 antibody
    • GRP-94 antibody
    • Heat shock protein 90 kDa beta member 1 antibody
    • heat shock protein 90kDa beta (Grp94), member 1 antibody
    • Heat shock protein, 90 kDa, beta, 1 antibody
    • HSP90B1 antibody
    • Stress inducible tumor rejection antigen GP96 antibody
    • TRA1 antibody
    • tumor rejection antigen (gp96) 1 antibody
    • Tumor rejection antigen 1 antibody
    • Tumor rejection antigen gp96 antibody
    • Tumor rejection antigen-1 (gp96) antibody
    see all

Anti-GRP94 antibody images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue
    using ab53075 at 1/50 dilution, with and without immunizing peptide.
  • All lanes : Anti-GRP94 antibody (ab53075) at 1/300 dilution

    Lane 1 : SKOV3 cell extract with no immunizing peptide
    Lane 2 : SKOV3 cell extract with immunizing peptide


    Predicted band size : 92 kDa
    Observed band size : 92 kDa


  • Performed under reducing conditions.

    Predicted band size : 92 kDa
    Observed band size : 92 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-GRP94 antibody (ab53075)

ab53075 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Prostate- BPH)
Loading amount 10 µg
Specification Prostate- BPH
Treatment 10uM SF for 24 hours
Gel Running Conditions Reduced Denaturing (10% gel, MOPS buffer)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Mar 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate)
Specification Prostate
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid Buffer
Permeabilization No
Blocking step 10% horse serum + 1% BSA in TBST as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"