Overview

  • Product nameAnti-GRPR antibody
    See all GRPR primary antibodies
  • Description
    Rabbit polyclonal to GRPR
  • Tested applicationsSuitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human GRPR.

    (Peptide available as ab39962.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Caco2 (Human colonic carcinoma) A549 (Human lung adenocarcinoma epithelial)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab39963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa).Can be blocked with Human GRPR peptide (ab39962).
IHC-P 1/100 - 1/1000.
IP Use a concentration of 5 µg/ml.

Target

  • FunctionReceptor for gastrin releasing peptide (GRP). This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Tissue specificityHighly expressed in pancreas. Also expressed in stomach, adrenal cortex and brain.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BB2 antibody
    • Bombesin BB2 receptor antibody
    • bombesin receptor 2 antibody
    • Gastrin Releasing Peptide Receptor antibody
    • Gastrin-releasing peptide receptor antibody
    • GRP preferring bombesin receptor antibody
    • GRP R antibody
    • GRP-preferring bombesin receptor antibody
    • GRP-R antibody
    • Grp/bombesin receptor antibody
    • Grpr antibody
    • GRPR_HUMAN antibody
    see all

Anti-GRPR antibody images

  • GRPR was immunoprecipitated using 0.5mg Caco2 whole cell extract, 5µg of Rabbit polyclonal to GRPR and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Caco2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab39963.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 53kDa; GRPR

  • All lanes : Anti-GRPR antibody (ab39963) at 1 µg/ml

    Lane 1 : Caco 2 whole cell lysate (ab3950)
    Lane 2 : A549 whole cell lysate (ab7910)

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    This protein is known to be glycosylated which could explain the difference between the predicted molecular weight of 43kDa according to Swiss Prot and our observed molecular weight of 53kDa.

References for Anti-GRPR antibody (ab39963)

This product has been referenced in:
  • Rybalov M  et al. PSMA, EpCAM, VEGF and GRPR as imaging targets in locally recurrent prostate cancer after radiotherapy. Int J Mol Sci 15:6046-61 (2014). IHC-P ; Human . Read more (PubMed: 24727373) »
  • Ananias HJ  et al. Application of (99m)Technetium-HYNIC(tricine/TPPTS)-Aca-Bombesin(7-14) SPECT/CT in prostate cancer patients: A first-in-man study. Nucl Med Biol 40:933-8 (2013). Read more (PubMed: 23891351) »

See all 2 Publications for this product

Product Wall

Merci pour votre requête.

Nous n'offrons malheureusement pas d'échantillon gratuit ou de petit volume pour des essais préliminaires. Notre politique est que si un anticorps ne marche pas comme spécifié dans ...

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Thats great news. Thank you for letting me know.

If you need any help in the future please do not hesitate to contact us again.

I wish you all the best with your research.

I would try using the cool down period for 5, 10 and 15 minutes as you have stated.
If you do not have access to normal goat serum then 3% BSA in TBST should work just as well. I would include this in the diluent of the primary antibody too.
...

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Thank you for that extra information.
Having reviewed the protocol used there are a few things I would suggest which may improve the results you are currently observing.

1. If you want to use serum to block we generally recommend using ...

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Thank you for contacting us and reporting the problems encountered in using anti-GRPR antibody ab39963. I am sorry to hear that this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and w...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Username

Dr. Kirk McManus

Verified customer

Submitted May 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (pancreas (human), kidney liver and embryo (mouse),)
Specification pancreas (human), kidney liver and embryo (mouse),
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization No
Blocking step sequential (prediluated) peroxidase and protein block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"