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Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human GRPR.
(Peptide available as ab39962.)
Our Abpromise guarantee covers the use of ab39963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa).Can be blocked with Human GRPR peptide (ab39962).|
|IHC-P||1/100 - 1/1000.|
|IP||Use a concentration of 5 µg/ml.|
GRPR was immunoprecipitated using 0.5mg Caco2 whole cell extract, 5µg of Rabbit polyclonal to GRPR and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Caco2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab39963.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 53kDa; GRPR