The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
FunctionMay play a role in actin filament architecture in developing stereocilia of sensory cells.
Tissue specificityExpressed at low levels in adult lung, brain and duodenum with moderate levels in testis. Highly expressed in fetal cochlea.
Involvement in diseaseDefects in GRXCR1 are the cause of deafness autosomal recessive type 25 (DFNB25) [MIM:613285]. DFNB25 is characterized by moderate to severe or profound hearing loss which is progressive in some individuals but not in others. Speech development is impaired in some but not all affected individuals and vestibular dysfunction is observed in some affected individuals.
Sequence similaritiesBelongs to the GRXCR1 family. Contains 1 glutaredoxin domain.
Cellular localizationCell projection > stereocilium. Cell projection > microvillus. Cell projection > kinocilium. In the inner ear, localized to stereocilia, apical microvilli of sensory cells and kinocilia.
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cell junctions. Recommended concentration of ab122670 1-4 µg/ml. Cells treated with PFA/Triton X-100.