Overview

  • Product nameAnti-GSC antibody
    See all GSC primary antibodies
  • Description
    Rabbit polyclonal to GSC
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (PALHGDSLYG ASGGASSDYG AFYPRPVAPG GAGLPAAVSG SRLGYNNYFY) of Human GSC (NP_776248).

  • Positive control
    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-GSC antibody images

  • Anti-GSC antibody (ab86968) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : ~32 kDa (why is the actual band size different from the predicted?)
    12% gel
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling GSC with ab86968 at 5.0µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling GSC with ab86968 at 5.0µg/ml.

References for Anti-GSC antibody (ab86968)

ab86968 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab86968.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"