Recombinant
RabMAb

Anti-GSC antibody [EPR4847] (ab109024)

Overview

  • Product name
    Anti-GSC antibody [EPR4847]
    See all GSC primary antibodies
  • Description
    Rabbit monoclonal [EPR4847] to GSC
  • Specificity
    Negative WB result with mouse brain, heart, kidney and spleen tissue lysates, but there is a feedback this antibody detected mouse GSC with NMuMG (mouse mammary gland, normal epithelial) cell lysate. Other samples has not been tested.
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human GSC.

  • Positive control
    • Human brain, HT29, MCF7, and HepG2 lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Regulates chordin (CHRD). May play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis. In concert with NKX3-2, plays a role in defining the structural components of the middle ear; required for the development of the entire tympanic ring.
    • Sequence similarities
      Belongs to the paired homeobox family. Bicoid subfamily.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Goosecoid antibody
      • goosecoid homeobox antibody
      • GSC antibody
      • GSC_HUMAN antibody
      • Homeobox protein goosecoid antibody
      see all

    Anti-GSC antibody [EPR4847] images

    • All lanes : Anti-GSC antibody [EPR4847] (ab109024) at 1/1000 dilution

      Lane 1 : Human brain lysate
      Lane 2 : HT29 lysate
      Lane 3 : MCF7 lysate
      Lane 4 : HepG2 lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 28 kDa

    References for Anti-GSC antibody [EPR4847] (ab109024)

    ab109024 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
    Gel Running Conditions
    Reduced Denaturing (Bis-Tris from Invitrogen 4-12%)
    Loading amount
    18 µg
    Specification
    Mouse embryonic stem cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Miss. Maud De Dieuleveult

    Verified customer

    Submitted Jul 04 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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