• Product nameAnti-GSC2 antibody
    See all GSC2 primary antibodies
  • Description
    Rabbit polyclonal to GSC2
  • Specificityab65032 detects endogenous levels of total GSC2 protein.
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human GSC2.

  • Positive control
    • 293, HepG2 and COLO205 cell extracts.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab65032 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab65032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 22 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • RelevanceGSC2, also known as Goosecoidlike or GSCL, is a homeodomain-containing gene that resides in the critical region for VCFS/DGS on 22q11. Velocardiofacial syndrome (VCFS) is a developmental disorder characterized by conotruncal heart defects, craniofacial anomalies, and learning disabilities and is phenotypically related to DiGeorge syndrome (DGS), both of which are associated with hemizygous 22q11 deletions.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • GSC 2 antibody
    • GSCL antibody
    • Homeobox protein goosecoid 2 antibody
    • Homeobox protein goosecoid like antibody

Anti-GSC2 antibody images

  • All lanes : Anti-GSC2 antibody (ab65032) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : HepG2 cell extract
    Lane 3 : COLO205 cell extract
    Lane 4 : COLO205 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 22 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,34 kDa. We are unsure as to the identity of these extra bands.
  • Immunostaining of mouse E9.5 embryo tissue sections. After using a biotinylated secondary, ABC and then DAB was used for color development. Please see abreview for protocol details.

    See Abreview

References for Anti-GSC2 antibody (ab65032)

ab65032 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount 50 µg
Specification Mouse ES Cells
Treatment Differentiating Conditions (Minus LIF Medium)
Gel Running Conditions Reduced Denaturing (15%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mr. Utkarsh Kapoor

Verified customer

Submitted Dec 31 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E9.5 embryo)
Specification E9.5 embryo
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

Dr. Jesse Mager

Verified customer

Submitted Feb 13 2009