Overview

  • Product name
  • Description
    Rabbit polyclonal to GSDMA
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human GSDMA aa 66-94 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • Mouse liver tissue lysates and Human skin carcinoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab104187 is antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 49 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    Function: May promote pyroptosis (Probable). Upon cleavage in vitro of genetically engineered GSDMA, the released N-terminal moiety binds to some types of lipids, such as possibly phosphatidylinositol (4,5)-bisphosphate. Homooligomerizes within the membrane and forms pores of 10 -15 nanometers (nm) of inner diameter, triggering cell death. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown. Tissue specificity: Expressed predominantly in the gastrointestinal tract and, at a lower level, in the skin. Also detected in mammary gland. In the gastrointestinal tract, mainly expressed in differentiated cells, including the differentiated cell layer of esophagus and mucus-secreting pit cells of the gastric epithelium. Down-regulateded in gastric cancer cells. Similarity: Belongs to the gasdermin family. Domain: Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain.
  • Cellular localization
    Cytoplasm, perinuclear region
  • Database links
  • Alternative names
    • FKSG9 antibody
    • Gasdermin 1 antibody
    • Gasdermin A antibody
    • Gasdermin-1 antibody
    • Gasdermin-A antibody
    • GSDM antibody
    • GSDM1 antibody
    • GSDMA antibody
    • GSDMA_HUMAN antibody
    see all

Images

  • Anti-GSDMA antibody (ab104187) at 1/100 dilution + mouse liver tissue lysates at 35 µg

    Predicted band size : 49 kDa
  • IHC analysis of GSDMA expression in formalin fixed and paraffin embedded Human skin carcinoma using 1/50 ab104187 followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab104187 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab104187.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up