Overview

  • Product nameAnti-GSG1L antibody
    See all GSG1L primary antibodies
  • Description
    Rabbit polyclonal to GSG1L
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    fragment corresponding to C terminal amino acids 257-331 (QGYREEPTFI DPEAIKYFRE RMEKRDGSEE DFHLDCRHER YPARHQPHMA DSWPRSSAQE APELNRQCWV LGHWV) of Human GSG1L.

  • Positive control
    • Human stomach tissue; RT-4, U-251 MG, liver and tonsil lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-GSG1L antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli and cytoskeleton (microtubules). Recommended concentration of ab121432 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121432 at a 1/75 dilution staining GSG1L in paraffin-embedded Human stomach tissue, by Immunohistochemistry.
  • All lanes : Anti-GSG1L antibody (ab121432) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique

References for Anti-GSG1L antibody (ab121432)

ab121432 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"