RabMAb

Anti-GSK3 (alpha + beta) antibody [15.4] (ab78277)

Overview

  • Product nameAnti-GSK3 (alpha + beta) antibody [15.4]See all GSK3 (alpha + beta) primary antibodies ...
  • Description
    Rabbit monoclonal [15.4] to GSK3 (alpha + beta)
  • Specificityab78277 recognizes both GSK3 alpha and beta.
  • Tested applicationsWB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length human GSK3 beta with N-terminal 6 His-tag, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

  • Positive control
    • A431, HEK293, Jurkat, and 3T3/A31 cell lysates. HeLa Nuclear lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab78277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: 2 - 8µl/100µg of lysate.
    WB: 1/1000 - 1/20000. Detects bands of approximately 47 and 51 kDa. Predicted molecular weights 47 kDa (beta) and 51 kDa (alpha).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceGlycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
    • Cellular localizationCytoplasmic and Nuclear
    • Database links
    • Alternative names
      • C86142 antibody
      • DKFZp686D0638 antibody
      • FA antibody
      • Factor A antibody
      • Glycogen Synthase Kinase 3 Alpha and Beta antibody
      • Glycogen synthase kinase 3 alpha antibody
      • Glycogen synthase kinase 3 beta antibody
      • GSK 3 alpha antibody
      • GSK 3 antibody
      • GSK 3 beta antibody
      • GSK 3A antibody
      • GSK 3B antibody
      • GSK3 alpha antibody
      • GSK3 beta antibody
      • Gsk3a antibody
      • GSK3B antibody
      • KG3A antibody
      • KG3B antibody
      see all

    Anti-GSK3 (alpha + beta) antibody [15.4] images

    • Anti-GSK3 (alpha + beta) antibody [15.4] (ab78277) at 1/5000 dilution + HEK293 cell lysate

      Secondary
      Goat anti-rabbit HRP conjugate

      Predicted band size : 47 , 51 kDa
      Observed band size : 47,51 kDa

    References for Anti-GSK3 (alpha + beta) antibody [15.4] (ab78277)

    ab78277 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"