Overview

  • Product nameAnti-GSK3 (alpha + beta) antibody [1H8]
    See all GSK3 (alpha + beta) primary antibodies
  • Description
    Mouse monoclonal [1H8] to GSK3 (alpha + beta)
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Monkey
  • Immunogen

    Recombinant Xenopus GSK3 beta protein.

  • Positive control
    • HeLa cell lysate (heat shocked), Mouse brain lysate, Rat brain lysate, RK-13 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000. Predicted molecular weight: 51 kDa (GSK3 alpha) and 47 kDa (GSK3 beta).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceGlycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
    • Cellular localizationCytoplasmic and Nuclear
    • Database links
    • Alternative names
      • Factor A antibody
      • Glycogen synthase kinase 3 alpha antibody
      • Glycogen synthase kinase 3 beta antibody
      • GSK3 alpha antibody
      • GSK3 beta antibody
      • GSK3B antibody
      see all

    Anti-GSK3 (alpha + beta) antibody [1H8] images

    • All lanes : Anti-GSK3 (alpha + beta) antibody [1H8] (ab90366) at 1/1000 dilution

      Lane 1 : HeLa lysate (heat shocked)
      Lane 2 : Mouse brain lysate
      Lane 3 : Rat brain lysate
      Lane 4 : RK-13 lysate

      Lysates/proteins at 20 µg per lane.


      Predicted band size : 51 , 47 kDa

    References for Anti-GSK3 (alpha + beta) antibody [1H8] (ab90366)

    This product has been referenced in:
    • Aza-Blanc P  et al. Identification of modulators of TRAIL-induced apoptosis via RNAi-based phenotypic screening. Mol Cell 12:627-37 (2003). Read more (PubMed: 14527409) »
    • Aza-Blanc P  et al. Identification of modulators of TRAIL-induced apoptosis via RNAi-based phenotypic screening. Mol Cell 12:627-37 (2003). WB . Read more (PubMed: 14527409) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293)
    Loading amount 20 µg
    Specification HEK293
    Gel Running Conditions Non-reduced Denaturing (10% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted May 17 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"