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Synthetic peptide (Human) (C terminal).
GSK3 (Glycogen Synthase Kinase-3 differs from most serine/threonine kinases) in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK-3a and GSK-3ß. The function of GSK-3 is to phosphorylate glycogen synthase and thereby inactivate it. Insulin action stimulates the PI 3-kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK-3.
Our Abpromise guarantee covers the use of ab15314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration. PubMed: 22003387|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.|
|IHC-P||Use at an assay dependent concentration. 1/100 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate, pH 6.0 for 10 min followed by cooling at RT for 20 min.|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"