Overview

  • Product nameAnti-GSK3 (alpha + beta) antibodySee all GSK3 (alpha + beta) primary antibodies ...
  • Description
    Rabbit polyclonal to GSK3 (alpha + beta)
  • Specificityab52179 detects endogenous levels of total GSK3a/ß protein.
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human GSK3a/ß around the phosphorylation site of tyrosine 279/216 (V-S-Y-I-C).

  • Positive control
    • Extracts from 293 cells, treated with TNF-a (20ng/ml, 30min).

Properties

Applications

Our Abpromise guarantee covers the use of ab52179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ELISA 1/20000.

Target

  • RelevanceGlycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • Factor A antibody
    • Glycogen synthase kinase 3 alpha antibody
    • Glycogen synthase kinase 3 beta antibody
    • GSK3 alpha antibody
    • GSK3 beta antibody
    • GSK3B antibody
    see all

Anti-GSK3 (alpha + beta) antibody images

  • All lanes : Anti-GSK3 (alpha + beta) antibody (ab52179) at 1/500 dilution

    Lane 1 : 293 cell extract, treated with TNF-a (20ng/ml, 30min)
    Lane 2 : 293 cell extract, treated with TNF-a (20ng/ml, 30min) with immunising peptide


    Predicted band size : 52 kDa
    Observed band size : 49,52 kDa (why is the actual band size different from the predicted?)

References for Anti-GSK3 (alpha + beta) antibody (ab52179)

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"