Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody (ab4797)

Overview

  • Product nameAnti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibodySee all GSK3 (alpha + beta) primary antibodies ...
  • Description
    Rabbit polyclonal to GSK3 (alpha + beta) (phospho Y216 + Y279)
  • SpecificityPhosphorylation site-specific antibody selective for the phosphorylated isoforms of glycogen synthase kinase enzyme, GSK 3 alpha, a 51 kDa protein containing a phosphate on tyrosine 279, and GSK 3 beta, a 47 kDa protein containing a phosphate on tyrosine 216.
  • Tested applicationsWB, Dot Blot, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from the regions of GSK 3 alpha + beta protein that contain tyrosine 216/279.

  • Positive control
    • Serum starve 3T3-L1 cells for 3 hours, then stimulate with 100 nM insulin or 100 ng/mL IGF 1 for 5 min.
  • General notes


    Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology. GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from rabbit serum by epitope specific affinity chromatography. Any reactivity towards the non-tyrosine phosphorylated GSK 3 alpha + beta protein has been eliminated through a series of preabsorption steps.
  • Primary antibody notes Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology. GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab4797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 51,47 kDa. Use at a concentration of 0.5 - 1.0 µg/ml. Predicted molecular weight: 51kDa for GSK 3 alpha and 47 kDa for GSK 3 beta.
Dot Blot Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceGlycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • C86142 antibody
    • DKFZp686D0638 antibody
    • FA antibody
    • Factor A antibody
    • Glycogen Synthase Kinase 3 Alpha and Beta antibody
    • Glycogen synthase kinase 3 alpha antibody
    • Glycogen synthase kinase 3 beta antibody
    • Glycogen synthase kinase 3 beta antibody
    • GSK 3 alpha antibody
    • GSK 3 antibody
    • GSK 3 beta antibody
    • GSK 3 beta antibody
    • GSK 3A antibody
    • GSK 3B antibody
    • GSK3 alpha antibody
    • GSK3 beta antibody
    • Gsk3a antibody
    • GSK3B antibody
    • KG3A antibody
    • KG3B antibody
    see all

Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody images



  • Predicted band size : 51,47 kDa


    Rat PC12 cells were serum-starved or stimulated with 100 ng/mL NGF for 10 minutes. Cell lysates were generated and the NGF stimulated cells were treated with alkaline phosphatase for 30 minutes at 30ºC. The proteins were resolved by SDS-PAGE and transferred to PVDF membrane. Membranes were incubated with 1µg/mL pan GSK3alpha/beta and 0.5 µg/mL of ab4797. The signal was detected using a Goat F(ab’)2 anti-Rabbit IgG Alkaline Phosphatase antibody at a 1/5000 dilution and the membrane was incubated with CDP-substrate using the WesternStarTM method (Tropix). The data show that the pan antibody recognizes both the alpha and beta form of GSK. In addition, there is strong basal phosphorylation of the tyrosine site, on alpha Y279 and beta site Y216. This is slightly enhanced by NGF treatment and fully dephosphorylated when extracts are treated with alkaline phosphatase.

References for Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody (ab4797)

This product has been referenced in:
  • Elali A  et al. Mild chronic cerebral hypoperfusion induces neurovascular dysfunction, triggering peripheral beta-amyloid brain entry and aggregation. Acta Neuropathol Commun 1:75 (2013). WB ; Mouse . Read more (PubMed: 24252187) »
  • Cheng X  et al. Gestational diabetes mellitus impairs Nrf2-mediated adaptive antioxidant defenses and redox signaling in fetal endothelial cells in utero. Diabetes 62:4088-97 (2013). Read more (PubMed: 23974919) »

See all 5 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - other (brain capillary membranes)
Loading amount 5 µg
Specification brain capillary membranes
Treatment Roche Diagnostics Complete Protease Inhibitor
Gel Running Conditions Reduced Denaturing (Invitrogen NuPage Bis-Tris 4-12%, 1 mm x 15 wells)
Blocking step Pierce Protein-free T20 (TBS) Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
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Abcam user community

Verified customer

Submitted Mar 02 2012

Application Western blot
Sample Human Cell lysate - whole cell (huh-7)
Loading amount 20 µg
Specification huh-7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Sep 29 2009

Application Western blot
Sample Human Cell lysate - whole cell (HEK293T)
Loading amount 40 µg
Specification HEK293T
Treatment X gene transfection for 24hrs
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Chien Hsin Lee

Verified customer

Submitted Sep 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"