Anti-GSK3 alpha antibody [6G2F1,6G2F2] (ab59955)

Overview

  • Product nameAnti-GSK3 alpha antibody [6G2F1,6G2F2]See all GSK3 alpha primary antibodies ...
  • Description
    Mouse monoclonal [6G2F1,6G2F2] to GSK3 alpha
  • Tested applicationsWB, ELISA, IHC-Fr, IHC-P more details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Ni-NTA purified truncated recombinant GSK3 alpha expressed in E.coli strain BL21 (DE3)

  • Positive control
    • A459 cell lysate (WB). Human skin and lung carcinomas (IHC-P).

Properties

Applications

Our Abpromise guarantee covers the use of ab59955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA 1/10000.
IHC-Fr 1/500 - 1/2000.
IHC-P Use at an assay dependent dilution.

Target

  • FunctionImplicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession P49840 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686D0638 antibody
    • Glycogen synthase kinase 3 alpha antibody
    • Glycogen synthase kinase-3 alpha antibody
    • GSK 3 alpha antibody
    • GSK 3A antibody
    • GSK-3 alpha antibody
    • GSK3A antibody
    • GSK3A_HUMAN antibody
    • GSK3alpha antibody
    • Serine/threonine protein kinase GSK3A antibody
    see all

Anti-GSK3 alpha antibody [6G2F1,6G2F2] images

  • All lanes : Anti-GSK3 alpha antibody [6G2F1,6G2F2] (ab59955) at 1/500 dilution

    Lane 1 : Molecular weight markers
    Lane 2 : Truncated GSK3 alpha recombinant protein
    Lane 3 : A549 cell lysate


    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • ab59955, at 1/500 dilution, staining human GSK3 alpha in lung carcinoma with DAB staining. Paraffin embedded tissue.
  • ab59955, at 1/500 dilution, staining human GSK3 alpha in skin carcinoma with DAB staining; Paraffin embedded tissue.

References for Anti-GSK3 alpha antibody [6G2F1,6G2F2] (ab59955)

ab59955 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"