Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [0.T.57] (ab33021)


  • Product nameAnti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [0.T.57]
    See all GSK3 (alpha + beta) primary antibodies
  • Description
    Mouse monoclonal [0.T.57] to GSK3 (alpha + beta) (phospho Y216 + Y279)
  • Tested applicationsSuitable for: WB, ICCmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Drosophila melanogaster
  • Immunogen

    Synthetic peptide: C-KQLLHGEPNVSYpICSRY conjugated to Ovalbumin, corresponding to the catalytic domain (between subdomain VII and VIII), amino acids 203 - 219 of Fruit fly (Drosophila melanogaster) GSK3 (alpha + beta)

  • Positive control
    • Jurkat lysate, NIH 3T3 lysate, A431 lysate and rat L6 lysate.



Our Abpromise guarantee covers the use of ab33021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesICC: Use at an assay dependent dilution.

    WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 52 kDa. ab33021 detects GSK3 (alpha + beta) in RIPA lysates from human Jurkat cells, mouse 3T3, human A431 and rat L6 cell lysates. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with ab33021 (0.5ug/ml). Proteins were visualized using a goat anti mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

    Is unsuitable for IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceGlycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
    • Cellular localizationCytoplasmic and Nuclear
    • Database links
    • Alternative names
      • Factor A antibody
      • Glycogen synthase kinase 3 alpha antibody
      • Glycogen synthase kinase 3 beta antibody
      • GSK3 alpha antibody
      • GSK3 beta antibody
      • GSK3B antibody
      see all

    References for Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [0.T.57] (ab33021)

    This product has been referenced in:
    • Dolma K  et al. Presenilin influences glycogen synthase kinase-3 ß (GSK-3ß) for kinesin-1 and dynein function during axonal transport. Hum Mol Genet N/A:N/A (2013). WB . Read more (PubMed: 24105467) »
    • Cole AR & Sutherland C Measuring GSK3 expression and activity in cells. Methods Mol Biol 468:45-65 (2008). Read more (PubMed: 19099245) »

    See all 2 Publications for this product

    Product Wall

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    Unfortunately, we don't have any other anti-GSK3 antibodies in our catalogue which is guaranteed to work in drosophila. In addition, the only 2 antibodies which are guaranteed to work in flow cytometry contain BS...

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    Thank you for contacting us.

    I can confirm that ab33021 contains no BSA only the following:
    Preservative: 0.05% Sodium Azide
    Constituents: 40% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4

    However, ab18893 co...

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