Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] (ab68476)


  • Product nameAnti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y]
    See all GSK3 (alpha + beta) primary antibodies
  • Description
    Rabbit monoclonal [EPR933Y] to GSK3 (alpha + beta) (phospho Y216 + Y279)
  • SpecificityThis antibody detects both GSK alpha phosphorylated on tyrosine 279 and GSK3 beta phosphorylyated on tyrosine 216.
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding tyrosine 279 in human GSK3 alpha and tyrosine 216 in human GSK beta.

  • Positive control
    • 293 cell lysates,human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab68476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 47-52 kDa (predicted molecular weight: 51 , 47 kDa).
IP 1/40.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • RelevanceGlycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
    • Cellular localizationCytoplasmic and Nuclear
    • Database links
    • Alternative names
      • Factor A antibody
      • Glycogen synthase kinase 3 alpha antibody
      • Glycogen synthase kinase 3 beta antibody
      • GSK3 alpha antibody
      • GSK3 beta antibody
      • GSK3B antibody
      see all

    Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] images

    • Human brain tissue stained with ab68476 at 1/100 dilution.
    • Dot Blot analysis of Lane 1: GSK3 (alpha + beta) (pY216 + pY279) phospho peptide and Lane 2: GSK3 (alpha + beta) non-phospho peptide labeling GSK3 (alpha + beta) (phospho Y216 + Y279) with ab68476 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

    • All lanes : Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] (ab68476) at 1/2000 dilution

      Lane 1 : 293 cell lysate, cells untreated.
      Lane 2 : 293 cell lysate, cells treated with AP.

      Lysates/proteins at 10 µg per lane.

      HRP conjugated Goat anti-rabbit at 1/2000 dilution

      Predicted band size : 51 , 47 kDa
      Observed band size : 47-52 kDa (why is the actual band size different from the predicted?)

    References for Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] (ab68476)

    This product has been referenced in:
    • Xu R  et al. The protease Omi regulates mitochondrial biogenesis through the GSK3ß/PGC-1a pathway. Cell Death Dis 5:e1373 (2014). WB ; Mouse . Read more (PubMed: 25118933) »
    • Weigand S  et al. Global quantitative phosphoproteome analysis of human tumor xenografts treated with a CD44 antagonist. Cancer Res 72:4329-39 (2012). Read more (PubMed: 22777824) »

    See all 4 Publications for this product

    Product Wall

    Application Immunohistochemistry free floating
    Sample Mouse Tissue sections (Brain)
    Specification Brain

    Abcam user community

    Verified customer

    Submitted Jan 14 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry free floating
    Sample Mouse Tissue sections (Hippocampus CA1 mouse Brain)
    Specification Hippocampus CA1 mouse Brain

    Abcam user community

    Verified customer

    Submitted Jan 19 2016

    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample Zebrafish Tissue sections (retina)
    Specification retina
    Permeabilization Yes - triton X
    Fixative Paraformaldehyde

    Dr. Ryan MacDonald

    Verified customer

    Submitted May 22 2014