Overview

  • Product name
    Anti-GSK3 alpha (phospho S21) antibody
    See all GSK3 alpha primary antibodies
  • Description
    Rabbit polyclonal to GSK3 alpha (phospho S21)
  • Tested applications
    Suitable for: ICC/IF, ELISA, WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    The antiserum was produced against synthesized phosphopeptide derived from human GSK3a around the phosphorylation site of Serine 21.

Properties

Applications

Our Abpromise guarantee covers the use of ab28808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 5 µg/ml.
ELISA Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

Images

  • Immunohistochemical analysis of paraffin embedded breast carcinoma, using primary CDK2 (Phospho-Thr160) ab28808 at 1:100 dilution and Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.
  • All lanes : Anti-GSK3 alpha (phospho S21) antibody (ab28808) at 1/1000 dilution

    Lane 1 : Extracts from ovary cancer cells.
    Lane 2 : Extracts from ovary cancer cells. with synthesised non phosphopeptide at 1 µg/ml
    Lane 3 : Extracts from ovary cancer cells. with synthesised phosphopeptide at 1 µg/ml

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • ICC/IF image of ab28808 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28808, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Qian CJ  et al. Notch4 inhibition reduces migration and invasion and enhances sensitivity to docetaxel by inhibiting Akt/fascin in pancreatic cancer cells. Oncol Lett 12:3499-3505 (2016). WB ; Human . Read more (PubMed: 27900027) »
  • Borghgraef P  et al. Increasing brain protein O-GlcNAc-ylation mitigates breathing defects and mortality of Tau.P301L mice. PLoS One 8:e84442 (2013). WB ; Mouse . Read more (PubMed: 24376810) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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