Anti-GSK3 alpha (phospho S21) antibody (ab9765)

Overview

  • Product name
    Anti-GSK3 alpha (phospho S21) antibody
    See all GSK3 alpha primary antibodies
  • Description
    Rabbit polyclonal to GSK3 alpha (phospho S21)
  • Specificity
    Specific for GSK 3 alpha isoform. Recognises a band at 52 kD.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: RARTSpSFAEPC, corresponding to amino acids 16-25 of Human GSK3 alpha.

Properties

Applications

Our Abpromise guarantee covers the use of ab9765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    Western blot: use at 1/1000.
    This product has been assayed by ELISA against 0.1µg of the immunizing peptide.
    A 1/1000 to 1/5000 dilution of the antibody is recommended for this assay.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.
  • Target

    • Function
      Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686D0638 antibody
      • Glycogen synthase kinase 3 alpha antibody
      • Glycogen synthase kinase-3 alpha antibody
      • GSK 3 alpha antibody
      • GSK 3A antibody
      • GSK-3 alpha antibody
      • Gsk3a antibody
      • GSK3A_HUMAN antibody
      • GSK3alpha antibody
      • Serine/threonine protein kinase GSK3A antibody
      see all

    Images

    • This image is courtesy of Angela Carter, Experimental Therapeutics, Ontario Cancer Inst, Toronto, Canada

      Rabbit polyclonal to phospho GSK alpha (Ser 21) at 1/1000 on 293T cell extract shows a band at 52 kD.
      Cells were serum starved for 24 hours:

      Lane 1 : control
      Lane 2 : treated with IGF-1 (100 ng/ml) for 20 min
      Lane 3 : pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20 min

      Rabbit polyclonal to phospho GSK alpha (Ser 21) at 1/1000 on 293T cell extract shows a band at 52 kD.
      Cells were serum starved for 24 hours:

      Lane 1 : control
      Lane 2 : treated with IGF-1 (100 ng/ml) for 20 min
      Lane 3 : pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20 min

    References

    ab9765 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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