Anti-GSK3 beta antibody (ab18893)

Overview

  • Product nameAnti-GSK3 beta antibody
    See all GSK3 beta primary antibodies
  • Description
    Rabbit polyclonal to GSK3 beta
  • SpecificityThe antibody detects GSK-3beta in samples from human, mouse and rat origin.
  • Tested applicationsSuitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Drosophila melanogaster
  • Immunogen

    Active recombinant full length protein (Human) GSK-3beta. (Peptide available as ab41719.)

Associated products

Applications

Our Abpromise guarantee covers the use of ab18893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 10 - 30 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 47 kDa.Can be blocked with GSK3 beta peptide (ab41719).

Target

  • FunctionParticipates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
  • Tissue specificityExpressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.
  • Cellular localizationCytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glycogen Synthase Kinase 3 Beta antibody
    • Glycogen synthase kinase-3 beta antibody
    • GSK 3 beta antibody
    • GSK-3 beta antibody
    • GSK3B antibody
    • GSK3B_HUMAN antibody
    • GSK3beta isoform antibody
    • Serine/threonine-protein kinase GSK3B antibody
    see all

References for Anti-GSK3 beta antibody (ab18893)

ab18893 has not yet been referenced specifically in any publications.

Product Wall

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Unfortunately, we don't have any other anti-GSK3 antibodies in our catalogue which is guaranteed to work in drosophila. In addition, the only 2 antibodies which are guaranteed to work in flow cytometry contain BS...

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I can confirm that ab33021 contains no BSA only the following:
Preservative: 0.05% Sodium Azide
Constituents: 40% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4

However, ab18893 co...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab32391.

To check the status of the order please contact o...

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Thank you for contacting us. ICC is Immunocytochemistry, this term can be used interchangeably with ICC/IF (Immunocytochemistry/ Immunofluorescence). This technique is specific to the fixed cultured cells. IHC; Immunohistochemistry; this is gen...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (fly extract)
Loading amount 25 µg
Specification fly extract
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

Submitted Oct 16 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"