Overview

  • Product nameAnti-GSK3 beta antibody [G8]See all GSK3 beta primary antibodies ...
  • Description
    Mouse monoclonal [G8] to GSK3 beta
  • SpecificityThis antibody is specific for GSK3 beta
  • Tested applicationsWB, IP, ELISA, ICC/IF more details
  • Species reactivity
    Reacts with: Rat, Human, Xenopus laevis
  • Immunogen

    E.coli produced recombinant Xenopus laevis GSK3 beta.

  • EpitopeThe exact epitope recognised by this antibody has not been mapped.

Applications

Our Abpromise guarantee covers the use of ab2602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000. Predicted molecular weight: 52 kDa.
IP Use at an assay dependent dilution.
Use at a concentration of 5 - 10 µg. The protein does not retain the kinase activity after immunoprecipitation.
ELISA 1/1000.
ICC/IF Use at an assay dependent dilution. PubMed: 19047045

Target

  • FunctionParticipates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
  • Tissue specificityExpressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.
  • Cellular localizationCytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Target information above from: UniProt accession P49841 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Glycogen Synthase Kinase 3 Beta antibody
    • Glycogen synthase kinase-3 beta antibody
    • GSK 3 beta antibody
    • GSK 3B antibody
    • GSK-3 beta antibody
    • GSK3B antibody
    • GSK3B protein antibody
    • GSK3B_HUMAN antibody
    • GSK3beta isoform antibody
    • Serine/threonine-protein kinase GSK3B antibody
    see all

Anti-GSK3 beta antibody [G8] images

  • ab2602 staining GSK3 beta in primary chondrocytes from the Sox9c/c mice after Cre-adenovirus infection by Immunocytochemistry/ Immunofluorescence.

References for Anti-GSK3 beta antibody [G8] (ab2602)

This product has been referenced in:
  • Dolma K  et al. Presenilin influences glycogen synthase kinase-3 ß (GSK-3ß) for kinesin-1 and dynein function during axonal transport. Hum Mol Genet N/A:N/A (2013). WB . Read more (PubMed: 24105467) »
  • Topol L  et al. Sox9 inhibits Wnt signaling by promoting beta-catenin phosphorylation in the nucleus. J Biol Chem 284:3323-33 (2009). WB, ICC/IF . Read more (PubMed: 19047045) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (A549)
Loading amount 20 µg
Specification A549
Treatment TRAIL
Gel Running Conditions Reduced Denaturing (12.5% SDS-PAGE)
Blocking step Odyssey as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 02 2012

Application Western blot
Sample Human Cell lysate - whole cell (293FT)
Loading amount 40 µg
Specification 293FT
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Chien Hsin Lee

Verified customer

Submitted Sep 11 2009

Thank you for your enquiry. The source of ab2602 replied to us; "In our hands we see only two bands. The weaker one, we believe is the GSK3-alpha. We have no evidence of its reactivity with GSK2-alpha." I hope this information helps, please do not ...

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I have just been informed that the anti-GSK monoclonal does NOT retain the kinase activity after the Immunoprecipitation. I hope this information helps, my apologies for the inconvenience,

My sincere apologies for the delay in responding to you, I have just this minute received this information from the source of ab2602: "This monoclonal antibody will work well in immunoprecipitation, however, its affect on the GSK3 kinase activity has ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"