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Synthetic phosphopeptide derived from human GSK3 beta around the phosphorylation site of tyrosine 216 (V-S-YP-I-C).
Our Abpromise guarantee covers the use of ab75745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22096577|
|ICC/IF||1/100 - 1/200.|
|WB||1/500 - 1/1000. Predicted molecular weight: 47 kDa.Can be blocked with GSK3 beta (phospho Y216) peptide (ab110532). This antibody can be used with studies using ab110532.|
|IHC-P||1/50 - 1/100.|
ab75745 at 1/100 dilution staining GSK3 beta in human Hela cells by ICC/IF.