Overview

  • Product nameGST Activity Assay Kit (Colorimetric)
    See all GST kits
  • Detection methodColorimetric
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • Assay typeEnzyme activity
  • Sensitivity
    < 4 mU/well
  • Assay time
    1h 30m
  • Species reactivity
    Reacts with: Human
    Predicted to work with: all Mammals
  • Product overview

    GST Activity Assay Kit (Colorimetric) (ab65326) is based upon the GST-catalyzed reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene, which has the broadest range of isozyme detectability (e.g., alpha-, mu-, pi-, and other GST isoforms). Under certain conditions, the interaction between glutathione and CDNB is totally dependent on the presence of active GST. The GST-catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected by spectrophotometer at 340 nm. One unit of GST activity is defined as the amount of enzyme producing 1 µmol of GS-DNB conjugate/min under the conditions of the assay.

    The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein. Detect limit: Active GST < 1 mU.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glutathione S-transferase (GST, EC 2.5.1.13) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • RelevanceGlutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta.
  • Cellular localizationCytoplasmic
  • Alternative names
    • Glutathione S transferase A1
    • Glutathione S transferase A2
    • GST class alpha 1
    • GST class alpha 2
    • GST epsilon
    • GST gamma
    • GSTA1
    • GSTA2
    • GTH1
    • GTH2
    • HA subunit 1
    • HA subunit 2
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab65326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

GST Activity Assay Kit (Colorimetric) images

  • GST Kinetic Assay performed according to the protocol.

  • GST activity calculated in cell lysates, with background signal subtracted (duplicates +/- SD), using equation in protocol.

  • GST activity levels in human saliva, mouse serum and mouse plasma samples. Interpolated values from two concentrations were multiplied by their dilution factor to represent the level in a neat sample. Background subtracted data is plotted.

Protocols

References for GST Activity Assay Kit (Colorimetric) (ab65326)

This product has been referenced in:
  • Anathy V  et al. Oxidative processing of latent Fas in the endoplasmic reticulum controls the strength of apoptosis. Mol Cell Biol 32:3464-78 (2012). Read more (PubMed: 22751926) »

See 1 Publication for this product

Product Wall



Ich möchte hiermit auch bestätigen, dass beide Kits für Zellkulturüberstand getestet und auch garantiert sind.

Sie haben Recht, dass eine Probenaufbereitung nicht gesondert erwähnt wird.

Zellkultur...

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Thank you for your enquiry and your interest in our products.

This kit is not specific for any GST isoforms so it detects the total GST enzymatic activity in the samples (using CDNB substrate).

There are some other GST substrates av...

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Thank you for your enquiries which have been forwarded to me as my colleague Karin is currently away from the office.

I am sorry to hear you have had some difficulties with this kit and for the inconvenience this has caused. As agreed with K...

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Thank you very much for your reply and reporting the moisture you have noticed with the GSH.

The lab are going to test some stock of this compound to investigate this solubility problem you have been experiencing further. I will get back to yo...

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Thank you for your reply.

I am glad to hear your subsequent runs with the kit have worked out well. I am still waiting on further information from the lab to advise you further in regards to the precipitate found in the GSH component and will...

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Thank you for your reply.

I have asked my colleagues in the US to look into if we can offer any discount on the order. One we can certainly offer free of charge as I offered to you previously. They should be in contact soon to arrange for the...

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Thank you for your reply.

I cannot currently find a reference to the "nmol" but it seems this may have been an error. The protocol booklet should read as in the attached document (with the GST activity units in μmol/min/ml).
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Sorry for the delay in my reply.

The units calculated for the GST activity should be in μmol/min/ml. The μmol unit comes from the extinction coefficient. I could not find any reference in out protocol to nmol. Where have you found this?...

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Sorry for the delay in getting back to you.

I now have feedback in regards to the calculations you are trying to perform:

The source of the kit has confirmed that thesecond of your calculations accordingis the closest to being corre...

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Thank you for getting back to me and sorry for the delay in my reply.

Hopefully I can now sort out dome of the confusing information. There has been an error in the equation provided to calculate GST. Unfortunately, the extinction coefficient...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"