Overview

  • Product name
    Anti-GST antibody [1E5]
    See all GST primary antibodies
  • Description
    Mouse monoclonal [1E5] to GST
  • Tested applications
    Suitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    not applicable
  • Immunogen

    Recombinant GST purified from E. coli

  • Positive control
    • recombinant GST and GST fusion protein

Properties

Applications

Our Abpromise guarantee covers the use of ab69186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
ELISA Use at an assay dependent concentration.
WB 1/500 - 1/2000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

  • Relevance
    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • Alternative names
    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Images

  • Immunofluorescence analysis of GST in HeLa cells using ab69186 at 1/100. Cells were counter-stained with DAPI (blue) for nuclear staining and a Alexa Fluor® 488 conjugated goat anti-mouse IgG was used as the secondary antibody (green).

  • All lanes : Anti-GST antibody [1E5] (ab69186) at 1/1000 dilution

    Lane 1 : GST recombinant protein
    Lane 2 : GST-fusion protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
    Additional bands at : 55 kDa (possible Fusion protein).

References

ab69186 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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